| Metadata details for analysis AN004942 | |
|---|---|
| Study ID | ST003011 |
| Analysis ID | AN004942 |
| Study Title | Untargeted metabolomics analysis of murine colon content |
| Institute | Helmholtz Centre for Environmental Research |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6546 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1290 Infinity II |
| Chromatography Type | Reversed phase |
| Chromatography Column | Agilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um) |
| Solvent A | Water + 0.1% FA |
| Solvent B | ACN + 0.1% FA |
| Gradient | 0-5.5 min: 1% B; 5-20 min: 1-100% B; 20-22 min: 100% B; 22-22.5: 100-1% B; 22.5-25 min: 1% B |
| Flow rate | 0.3 ml/min |
| Column Temperature | 45 |
| Retention time units | Minutes |