| Metadata details for analysis AN004951 | |
|---|---|
| Study ID | ST003017 |
| Analysis ID | AN004951 |
| Study Title | Lipidomics and plasma hormone reveal indicators of reproductive status in Florida manatees (Trichechus manatus latirostris) |
| Institute | University of Florida |
| Species | Florida manatee (Trichechus manatus latirostris) |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) |
| Solvent A | 60:40 acetonitrile/water (v/v), 5mM ammonium formate and 0.1% formic acid |
| Solvent B | 90:10 isopropanol/acetonitrile (v/v), 5mM ammonium formate and 0.1% formic acid |
| Gradient | a linear increase of solvent B from 40% to 55% over 7 min, then increased to 65% by min 8, held at 65% for 4 min, linear increase to 95% over 8 min, increased to 100% over 2 min, solvent B was held at 100% for 5 min before decreasing to 40% in a tenth of a min. Finally, solvent B was held at 100% for another 3 min. |
| Flow rate | 0.25 mL/min |
| Column Temperature | 45 |
| Retention time units | Seconds |
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