| Metadata details for analysis AN005042 | |
|---|---|
| Study ID | ST003084 |
| Analysis ID | AN005042 |
| Study Title | Metabolome changes in embryonic CSF (Part 6) |
| Institute | Boston Children's Hospital, Harvard Medical School |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | SeQuant ZIC- pHILIC (150 x 2.1mm, 5um) |
| Solvent A | 100% acetonitrile |
| Solvent B | 100% water; 20mM ammonium carbonate, 0.1% ammonium hydroxide |
| Gradient | 0-20 min: linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B. |
| Flow rate | 0.150mL/minute |
| Column Temperature | 25 |
| Retention time units | Minutes |
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