| Metadata details for analysis AN005072 | |
|---|---|
| Study ID | ST003101 |
| Analysis ID | AN005072 |
| Study Title | Parallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens |
| Institute | Life Sciences Institute, The University of British Columbia |
| Species | Apis mellifera |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Bruker Impact II |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% methanol; 0.1% formic acid |
| Gradient | 0 min (5% B), 0–1 min (5% B), 1–8 min (35% B), 8–10.5 min (99% B), 10.5–14 min (99% B), 14–14.5 min (5% B), and 14.5–18 min (5% B) |
| Flow rate | 0.3 ml/min |
| Column Temperature | 55 |
| Retention time units | Minutes |
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