| Metadata details for analysis AN005074 | |
|---|---|
| Study ID | ST003101 |
| Analysis ID | AN005074 |
| Study Title | Parallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens |
| Institute | Life Sciences Institute, The University of British Columbia |
| Species | Apis mellifera |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Bruker Impact II |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| Solvent A | 100% water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
| Gradient | 0 min (20% B), 0–2 min (20% B), 2–11 min (80% B), 11–11.5 min (99% B), 11.5–13.2 min (99% B), 13.2–14 min (5% B), and 14–18 min (5% B). |
| Flow rate | 0.4 ml/min |
| Column Temperature | 65 |
| Retention time units | Minutes |
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