| Metadata details for analysis AN005114 | |
|---|---|
| Study ID | ST003120 |
| Analysis ID | AN005114 |
| Study Title | Mannose is crucial for mesoderm specification and symmetry breaking in gastruloids. |
| Institute | Dept of Genetics, University of Cambridge |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Fisher Scientific Orbitrap Exploris 240 |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Vanquish UHPLC |
| Chromatography Type | HILIC |
| Chromatography Column | Atlantis Premier BEH Z-HILIC (100 x 2.1 mm, 1.7 um) |
| Solvent A | water:acetonitrile 9:1, v/v; 10mM ammonium acetate |
| Solvent B | acetonitrile:water 9:1, v/v; 10mM ammonium acetate |
| Gradient | time [min]/%B - 0/95, 2/95, 14.5/60, 16/60, 16.5/95, 20/95 |
| Flow rate | 0.25mL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
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