| Metadata details for analysis AN005235 | |
|---|---|
| Study ID | ST003187 |
| Analysis ID | AN005235 |
| Study Title | Untargeted plasma metabolomics on bacterial culture supernatants |
| Institute | Harvard University |
| Species | Escherichia coli |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Agilent 6530 QTOF |
| MS Instrument Type | QTOF |
| Chromatography Instrument Name | Agilent 1260 Infinity HPLC |
| Chromatography Type | HILIC |
| Chromatography Column | Waters Atlantis HILIC (150 x 2.1mm,3um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | 0.5 minute at 5% mobile phase B followed by a linear gradient to 40% mobile phase B over 10 minutes and then maintained isocratically for 4.5 minutes before returning to 5% mobile phase B by gradient over 2 minutes |
| Flow rate | 250 μL/min |
| Column Temperature | 30 |
| Retention time units | Minutes |