Metadata details for analysis AN005398
Study IDST003294
Analysis IDAN005398
Study TitleExploring the Effects of Toxic Road Runoff Contaminants (6PPD-quinone, 9,10-anthraquinone) on Juvenile Salmonids with a Multi-OMICs Approach (Brain)
InstituteOregon State University
SpeciesOncorhynchus tshawytscha, Oncorhynchus mykiss, Oncorhynchus kisutch
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameABI Sciex 7600 ZenoTOF
MS Instrument TypeQTOF
Chromatography Instrument NameSciex ExionLC AD
Chromatography TypeReversed phase
Chromatography ColumnGL Sciences Intersil Ph-3 (150 x 2.1 mm, 2µm)
Solvent A100% Water; 0.1% Formic acid
Solvent B100% Acetonitrile; 0.1% Formic acid
GradientIn %B, at minute 0, 5% B, and held at 5% B until minute 3. From 3-12 minutes a gradient up to 98% B and held at 98% until 17 minutes. From 17 to 17.5 drop to 5% B and held at 5% until 23 minutes.
Flow rate0.3 mL/min
Column Temperature40℃
Retention time unitsMinutes
Study IDST003294
Analysis IDAN005398
Study TitleExploring the Effects of Toxic Road Runoff Contaminants (6PPD-quinone, 9,10-anthraquinone) on Juvenile Salmonids with a Multi-OMICs Approach (Brain)
InstituteOregon State University
SpeciesOncorhynchus tshawytscha, Oncorhynchus mykiss, Oncorhynchus kisutch
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameABI Sciex 7600 ZenoTOF
MS Instrument TypeQTOF
Chromatography Instrument NameSciex ExionLC AD
Chromatography TypeReversed phase
Chromatography ColumnGL Sciences Intersil Ph-3 (150 x 2.1 mm, 2µm)
Solvent A100% Water; 0.1% Formic acid
Solvent B100% Acetonitrile; 0.1% Formic acid
GradientIn %B, at minute 0, 5% B, and held at 5% B until minute 3. From 3-12 minutes a gradient up to 98% B and held at 98% until 17 minutes. From 17 to 17.5 drop to 5% B and held at 5% until 23 minutes.
Flow rate0.3 mL/min
Column Temperature40℃
Retention time unitsMinutes
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