Metadata details for analysis AN005447
Study IDST003325
Analysis IDAN005447
Study TitleComprehensive Untargeted LC-MS Metabolomics Analysis of STS Knockout iPSCs(Supernatant)
InstituteThe Jackson Laboratory for Genomic Medicine
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF-X Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish Duo UHPLC
Chromatography TypeReversed phase
Chromatography ColumnWaters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Solvent A95% water/5% acetonitrile; 0.1% Formic acid
Solvent B100% acetonitrile; 0.1% Formic acid (v/v)
Gradient0.0min: 0%B, 1.0min: 0%B, 4.0min: 30%B, 17.0min: 100%B, 20.0min: 100%B, 20.5min: 0%B, 25.0min: 0%B.
Flow rate0.4 mL/min
Column Temperature45
Retention time unitsSeconds
Study IDST003325
Analysis IDAN005447
Study TitleComprehensive Untargeted LC-MS Metabolomics Analysis of STS Knockout iPSCs(Supernatant)
InstituteThe Jackson Laboratory for Genomic Medicine
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF-X Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish Duo UHPLC
Chromatography TypeReversed phase
Chromatography ColumnWaters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Solvent A95% water/5% acetonitrile; 0.1% Formic acid
Solvent B100% acetonitrile; 0.1% Formic acid (v/v)
Gradient0.0min: 0%B, 1.0min: 0%B, 4.0min: 30%B, 17.0min: 100%B, 20.0min: 100%B, 20.5min: 0%B, 25.0min: 0%B.
Flow rate0.4 mL/min
Column Temperature45
Retention time unitsSeconds
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