Data for ST002124
| Metabolite structure | All data | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 | F14 | F15 | F16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| [13C, 15N]Alanine, 2TBDMS | 52266.67 | 5226666.67 | 4513333.33 | 4890000.00 | 66633.33 | 6663333.33 | 4943333.33 | 5763333.33 | 55800.00 | 5580000.00 | 4743333.33 | 5163333.33 | 48533.33 | 4853333.33 | 4216666.67 | 4550000.00 | |
| [13C, 15N]Aspartic acid, 3TBDMS | 74600.00 | 7460000.00 | 7066666.67 | 7223333.33 | 89033.33 | 8903333.33 | 3426666.67 | 5426666.67 | 79600.00 | 7960000.00 | 7460000.00 | 7656666.67 | 72566.67 | 7256666.67 | 6946666.67 | 7126666.67 | |
| [13C, 15N]Glutamic acid, 3TBDMS | 13166.67 | 1316666.67 | 1066666.67 | 1226666.67 | 27666.67 | 2766666.67 | 561666.67 | 1633333.33 | 16166.67 | 1616666.67 | 1113333.33 | 1426666.67 | 12033.33 | 1203333.33 | 995333.33 | 1063333.33 | |
| [13C, 15N]Glycine, 2TBDMS | 51900.00 | 3610000.00 | 3150000.00 | 3776666.67 | 59800.00 | 5670000.00 | 2823333.33 | 4050000.00 | 37566.67 | 4830000.00 | 3756666.67 | 4226666.67 | 46266.67 | 3266666.67 | 3360000.00 | 3420000.00 | |
| [13C, 15N]Histidine, 3TBDMS | 25833.33 | 2583333.33 | 2336666.67 | 2453333.33 | 33466.67 | 3346666.67 | 969666.67 | 2303333.33 | 25433.33 | 2543333.33 | 2296666.67 | 2383333.33 | 29400.00 | 2940000.00 | 2746666.67 | 2830000.00 | |
| [13C, 15N]Isoleucine, 2TBDMS | 91200.00 | 9120000.00 | 6913333.33 | 8130000.00 | 113833.33 | 11383333.33 | 3540000.00 | 6590000.00 | 94800.00 | 9480000.00 | 6890000.00 | 8256666.67 | 83466.67 | 8346666.67 | 6540000.00 | 8016666.67 | |
| [13C, 15N]Leucine, 2TBDMS | 93700.00 | 9370000.00 | 8623333.33 | 9093333.33 | 127000.00 | 12700000.00 | 4363333.33 | 8346666.67 | 93100.00 | 9310000.00 | 8873333.33 | 9030000.00 | 90133.33 | 9013333.33 | 8230000.00 | 8723333.33 | |
| [13C, 15N]Lysine, 3TBDMS | 51066.67 | 5106666.67 | 4286666.67 | 4540000.00 | 65633.33 | 6563333.33 | 3200000.00 | 5380000.00 | 49633.33 | 4963333.33 | 4360000.00 | 4636666.67 | 48266.67 | 4826666.67 | 4240000.00 | 4493333.33 | |
| [13C, 15N]Methionine, 2TBDMS | 84333.33 | 8433333.33 | 8016666.67 | 8180000.00 | 126533.33 | 12653333.33 | 7136666.67 | 8550000.00 | 99966.67 | 9996666.67 | 8593333.33 | 9370000.00 | 81700.00 | 8170000.00 | 7853333.33 | 7876666.67 | |
| [13C, 15N]Phenylalanine, 2TBDMS | 99533.33 | 9953333.33 | 8256666.67 | 8416666.67 | 128333.33 | 12833333.33 | 4926666.67 | 8153333.33 | 116666.67 | 11666666.67 | 7670000.00 | 8750000.00 | 83933.33 | 8393333.33 | 7943333.33 | 8136666.67 | |
| [13C, 15N]Proline, 2TBDMS | 46766.67 | 4676666.67 | 3816666.67 | 4230000.00 | 79666.67 | 7966666.67 | 2553333.33 | 5506666.67 | 52833.33 | 5283333.33 | 4006666.67 | 4286666.67 | 48400.00 | 4840000.00 | 4213333.33 | 4553333.33 | |
| [13C, 15N]Serine, 3TBDMS | 87933.33 | 8793333.33 | 8063333.33 | 8420000.00 | 92666.67 | 9266666.67 | 6610000.00 | 7816666.67 | 93366.67 | 9336666.67 | 8053333.33 | 8830000.00 | 88366.67 | 8836666.67 | 8366666.67 | 8526666.67 | |
| [13C, 15N]Threonine, 3TBDMS | 12266.67 | 1226666.67 | 1002000.00 | 1126666.67 | 17733.33 | 1773333.33 | 856333.33 | 1163333.33 | 12933.33 | 1293333.33 | 1053333.33 | 1173333.33 | 12333.33 | 1233333.33 | 1043000.00 | 1123333.33 | |
| [13C, 15N]Tyrosine, 3TBDMS | 314333.33 | 31433333.33 | 26466666.67 | 29800000.00 | 486333.33 | 48633333.33 | 21833333.33 | 32666666.67 | 320000.00 | 32000000.00 | 28300000.00 | 30366666.67 | 324333.33 | 32433333.33 | 28700000.00 | 30133333.33 | |
| [13C, 15N]Valine, 2TBDMS | 329000.00 | 32900000.00 | 22366666.67 | 23966666.67 | 461000.00 | 46100000.00 | 15566666.67 | 28300000.00 | 334666.67 | 33466666.67 | 23400000.00 | 23900000.00 | 323333.33 | 32333333.33 | 23566666.67 | 22600000.00 | |
| [13C]Alpha_Ketoglutarate | - | 18066.67 | 21266.67 | 19500.00 | - | 27633.33 | 76900.00 | 44033.33 | - | 18700.00 | 24133.33 | 21733.33 | - | 18866.67 | 26133.33 | 22733.33 | |
| [13C]Citrate | - | 45666.67 | 60766.67 | 53100.00 | - | 57766.67 | 199333.33 | 116000.00 | - | 45133.33 | 53733.33 | 50333.33 | - | 38300.00 | 56866.67 | 49200.00 | |
| [13C]Fumatate | - | 187666.67 | 208666.67 | 201000.00 | - | 205333.33 | 305333.33 | 254333.33 | - | 177333.33 | 201333.33 | 188666.67 | - | 189666.67 | 203333.33 | 194333.33 | |
| [13C]Malate | - | 68133.33 | 77433.33 | 74233.33 | - | 86500.00 | 121333.33 | 93666.67 | - | 69566.67 | 78866.67 | 73500.00 | - | 61333.33 | 77366.67 | 70233.33 | |
| [13C]Succinate | - | 135000.00 | 160333.33 | 146666.67 | - | 159333.33 | 268000.00 | 211333.33 | - | 133666.67 | 172666.67 | 149333.33 | - | 125333.33 | 164333.33 | 146000.00 | |
| Alanine, 2TBDMS | 5530000.00 | - | - | - | 1436666.67 | - | - | - | 4233333.33 | - | - | - | 6320000.00 | - | - | - | |
| Alpha_Ketoglutarate | 6300000.00 | - | - | - | 4176666.67 | - | - | - | 5936666.67 | - | - | - | 6213333.33 | - | - | - | |
| Aspartic acid, 3TBDMS | 769000000.00 | - | - | - | 118000000.00 | - | - | - | 627000000.00 | - | - | - | 1062333333.33 | - | - | - | |
| Citrate | 13400000.00 | - | - | - | 7803333.33 | - | - | - | 11300000.00 | - | - | - | 12633333.33 | - | - | - | |
| Fumatate | 64300000.00 | - | - | - | 41633333.33 | - | - | - | 62866666.67 | - | - | - | 65333333.33 | - | - | - | |
| Glutamic acid, 3TBDMS | 913666666.67 | - | - | - | 239666666.67 | - | - | - | 670000000.00 | - | - | - | 1150000000.00 | - | - | - | |
| Glutamine, 3TBDMS | 2226666.67 | - | - | - | 427333.33 | - | - | - | 1380000.00 | - | - | - | 3113333.33 | - | - | - | |
| Glycine, 2TBDMS | 1480000000.00 | - | - | - | 595666666.67 | - | - | - | 1193333333.33 | - | - | - | 1523333333.33 | - | - | - | |
| Histidine, 3TBDMS | 840000.00 | - | - | - | 172666.67 | - | - | - | 532000.00 | - | - | - | 1163333.33 | - | - | - | |
| Hydroxyproline, (E)-, 3TBDMS | 9813333.33 | - | - | - | 2066666.67 | - | - | - | 7340000.00 | - | - | - | 14233333.33 | - | - | - | |
| Isoleucine, 2TBDMS | 22300000.00 | - | - | - | 5736666.67 | - | - | - | 16366666.67 | - | - | - | 25466666.67 | - | - | - | |
| L-Cysteine, 3TBDMS | 8843333.33 | - | - | - | 1284000.00 | - | - | - | 5300000.00 | - | - | - | 12733333.33 | - | - | - | |
| Leucine, 2TBDMS | 89733333.33 | - | - | - | 24433333.33 | - | - | - | 67500000.00 | - | - | - | 98366666.67 | - | - | - | |
| Lysine, 3TBDMS | 50466666.67 | - | - | - | 11473333.33 | - | - | - | 40766666.67 | - | - | - | 62533333.33 | - | - | - | |
| Malate | 19200000.00 | - | - | - | 10066666.67 | - | - | - | 16266666.67 | - | - | - | 19500000.00 | - | - | - | |
| Methionine, 2TBDMS | 15900000.00 | - | - | - | 3513333.33 | - | - | - | 8760000.00 | - | - | - | 19100000.00 | - | - | - | |
| Phenylalanine, 2TBDMS | 38400000.00 | - | - | - | 6340000.00 | - | - | - | 26800000.00 | - | - | - | 46100000.00 | - | - | - | |
| Proline, 2TBDMS | 148000000.00 | - | - | - | 32266666.67 | - | - | - | 93766666.67 | - | - | - | 221666666.67 | - | - | - | |
| Serine, 3TBDMS | 317000000.00 | - | - | - | 63900000.00 | - | - | - | 241000000.00 | - | - | - | 345000000.00 | - | - | - | |
| Succinate | 64233333.33 | - | - | - | 42066666.67 | - | - | - | 56666666.67 | - | - | - | 65333333.33 | - | - | - | |
| Threonine, 3TBDMS | 11900000.00 | - | - | - | 2810000.00 | - | - | - | 7420000.00 | - | - | - | 18400000.00 | - | - | - | |
| Tyrosine, 3TBDMS | 3210000.00 | - | - | - | 625666.67 | - | - | - | 2790000.00 | - | - | - | 4343333.33 | - | - | - | |
| Valine, 2TBDMS | 54033333.33 | - | - | - | 14333333.33 | - | - | - | 39766666.67 | - | - | - | 55766666.67 | - | - | - |
Factors:
| F1 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F2 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F3 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F4 | Source_Name[gating]:Lineage-negative (Lin–) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F5 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F6 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F7 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F8 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| F9 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F10 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F11 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F12 | Source_Name[gating]:Lin– Scal1+ c-Kit+ (LSK) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F13 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 1 h | Batch:1a | Note:[13C, 15N] AAs were used for AA uptake (Fig1.B); unlabeled AAs were used for total AA (Fig1.A) and TCA substrate levels (Fig1.D). |
| F14 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F15 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
| F16 | Source_Name[gating]:Mature hematopoietic cells (Lin+ cells) | Treatment:[13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C). |
