Data for ST002125   

(Analysis AN003478): Average values per metabolite and experimental factor (Units:Peak Area)

Metabolite structureAll dataF1F2F3F4F5F6F7F8F9F10F11F12F13F14
[13C, 15N]Alanine, 2TBDMS
- - 67833.33 6783333.33 6163333.33 5603333.33 67500.00 6415000.00 6163333.33 5575000.00 6663333.33 4943333.33 5763333.33 61633.33
[13C, 15N]Aspartic acid, 3TBDMS
- - 112433.33 11243333.33 4600000.00 6190000.00 114666.67 10010000.00 4430000.00 5350000.00 8903333.33 3426666.67 5426666.67 78666.67
[13C, 15N]Glutamic acid, 3TBDMS
- - 72833.33 7283333.33 2296666.67 3880000.00 69400.00 6030000.00 1710000.00 3195000.00 2766666.67 561666.67 1633333.33 32966.67
[13C, 15N]Glycine, 2TBDMS
- - 78533.33 6770000.00 4310000.00 5260000.00 76833.33 6602500.00 4653333.33 4965000.00 5670000.00 2823333.33 4050000.00 57933.33
[13C, 15N]Histidine, 3TBDMS
- - 49333.33 4933333.33 3606666.67 4146666.67 47933.33 4660000.00 3296666.67 3930000.00 3346666.67 969666.67 2303333.33 40200.00
[13C, 15N]Isoleucine, 2TBDMS
- - 169666.67 16966666.67 10350000.00 14466666.67 163000.00 16025000.00 12533333.33 14350000.00 11383333.33 3540000.00 6590000.00 132666.67
[13C, 15N]Leucine, 2TBDMS
- - 199666.67 19966666.67 8636666.67 13900000.00 222000.00 19650000.00 7853333.33 13750000.00 12700000.00 4363333.33 8346666.67 134666.67
[13C, 15N]Lysine, 3TBDMS
- - 79200.00 7920000.00 3886666.67 5540000.00 83533.33 7655000.00 3863333.33 4945000.00 6563333.33 3200000.00 5380000.00 55300.00
[13C, 15N]Methionine, 2TBDMS
- - 183000.00 18300000.00 12266666.67 14666666.67 160333.33 15625000.00 11100000.00 12600000.00 12653333.33 7136666.67 8550000.00 141333.33
[13C, 15N]Phenylalanine, 2TBDMS
- - 133000.00 13300000.00 6236666.67 9693333.33 136000.00 12612500.00 6963333.33 10800000.00 12833333.33 4926666.67 8153333.33 115000.00
[13C, 15N]Proline, 2TBDMS
- - 136000.00 13600000.00 8343333.33 10900000.00 155666.67 14975000.00 7670000.00 12000000.00 7966666.67 2553333.33 5506666.67 84433.33
[13C, 15N]Serine, 3TBDMS
- - 129000.00 12900000.00 8016666.67 10153333.33 138333.33 13250000.00 7796666.67 10850000.00 9266666.67 6610000.00 7816666.67 79800.00
[13C, 15N]Threonine, 3TBDMS
- - 18733.33 1873333.33 1083333.33 1493333.33 19266.67 1832500.00 1153333.33 1605000.00 1773333.33 856333.33 1163333.33 13733.33
[13C, 15N]Tyrosine, 3TBDMS
- - 833000.00 83300000.00 39500000.00 61766666.67 849666.67 78325000.00 32066666.67 60900000.00 48633333.33 21833333.33 32666666.67 507666.67
[13C, 15N]Valine, 2TBDMS
- - 62933.33 62933333.33 43166666.67 54266666.67 70833.33 63925000.00 39166666.67 52300000.00 46100000.00 15566666.67 28300000.00 41900.00
[13C]Alpha_Ketoglutarate
- - 6246666.67 18133.33 20733.33 20466.67 6213333.33 15300.00 18333.33 17200.00 27633.33 76900.00 44033.33 4103333.33
[13C]Citrate
- - 14600000.00 31733.33 45666.67 37666.67 16233333.33 33900.00 41166.67 36250.00 57766.67 199333.33 116000.00 8053333.33
[13C]Fumatate
- - 62266666.67 122333.33 145000.00 129000.00 65133333.33 140500.00 157000.00 157500.00 205333.33 305333.33 254333.33 41066666.67
[13C]Malate
- - 22833333.33 65300.00 76133.33 73500.00 23600000.00 68075.00 76100.00 71250.00 86500.00 121333.33 93666.67 10453333.33
[13C]Succinate
- - 64633333.33 104200.00 131333.33 120000.00 64566666.67 103425.00 139333.33 122500.00 159333.33 268000.00 211333.33 41833333.33
Alanine, 2TBDMS
318333333.33 78000000.00 5043333.33 - - - 5400000.00 - - - - - - 1413333.33
Alpha_Ketoglutarate
- - 6246666.67 - - - 6213333.33 - - - - - - 4103333.33
Aspartic acid, 3TBDMS
3186666666.67 837666666.67 142000000.00 - - - 143333333.33 - - - - - - 44966666.67
Citrate
- - 14600000.00 - - - 16233333.33 - - - - - - 8053333.33
Fumatate
- - 62266666.67 - - - 65133333.33 - - - - - - 41066666.67
Glutamic acid, 3TBDMS
3780000000.00 1743333333.33 149333333.33 - - - 172333333.33 - - - - - - 42000000.00
Glutamine, 3TBDMS
365666666.67 151666666.67 1706666.67 - - - 1576666.67 - - - - - - 410000.00
Glycine, 2TBDMS
13866666666.67 3090000000.00 580000000.00 - - - 470666666.67 - - - - - - 240000000.00
Histidine, 3TBDMS
221666666.67 76733333.33 274000.00 - - - 248666.67 - - - - - - 48966.67
Hydroxyproline, (E)-, 3TBDMS
97566666.67 40000000.00 5100000.00 - - - 5006666.67 - - - - - - 1273333.33
Isoleucine, 2TBDMS
2120000000.00 433333333.33 17066666.67 - - - 16800000.00 - - - - - - 5290000.00
L-Cysteine, 3TBDMS
14500000.00 2883333.33 3063333.33 - - - 3250000.00 - - - - - - 771333.33
Leucine, 2TBDMS
1090000000.00 611666666.67 43866666.67 - - - 41700000.00 - - - - - - 13333333.33
Lysine, 3TBDMS
2143333333.33 472333333.33 16066666.67 - - - 13533333.33 - - - - - - 3940000.00
Malate
- - 22833333.33 - - - 23600000.00 - - - - - - 10453333.33
Methionine, 2TBDMS
1196666666.67 296666666.67 13300000.00 - - - 14466666.67 - - - - - - 3330000.00
Phenylalanine, 2TBDMS
2276666666.67 424333333.33 5720000.00 - - - 6930000.00 - - - - - - 2020000.00
Proline, 2TBDMS
1536666666.67 418666666.67 70233333.33 - - - 75000000.00 - - - - - - 11966666.67
Serine, 3TBDMS
3256666666.67 839666666.67 53300000.00 - - - 59966666.67 - - - - - - 20433333.33
Succinate
- - 64633333.33 - - - 64566666.67 - - - - - - 41833333.33
Threonine, 3TBDMS
328000000.00 97066666.67 7396666.67 - - - 6806666.67 - - - - - - 2506666.67
Tyrosine, 3TBDMS
4636666666.67 1566666666.67 20333333.33 - - - 23433333.33 - - - - - - 5626666.67
Valine, 2TBDMS
3323333333.33 890333333.33 48533333.33 - - - 46966666.67 - - - - - - 12866666.67

Factors:

F1Source_Name[gating]:Bone marrow supernatant | Treatment:5 FU 2 weeks | Batch:1c | Note:Unlabeled AAs were used for total AA (FigS2.D)
F2Source_Name[gating]:Bone marrow supernatant | Treatment:HSC Basal | Batch:1c | Note:Unlabeled AAs were used for total AA (FigS2.D)
F3Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J).
F4Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F5Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F6Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F7Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C) and TCA substrate levels (Fig2.K).
F8Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F9Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F10Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D).
F11Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D).
F12Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D).
F13Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1b | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D).
F14Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J).
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