Clustering data with hclust algorithm for Determining the metabolic profile of wildtype and nos mutant Staphylococcus aureus grown in media lacking glucose using targeted LC/MS (Study ST000247)
ESI positive ion mode Amino Acids(Instrument:Agilent 6490) (Analysis AN000390)| Metabolite | Structure | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 | F13 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Asparagine | ME700693 | 4.55 | 0.55 | 0.33 | 0.82 | 0.16 | 0.73 | 0.29 | 4.02 | 0.07 | 0.50 | 0.16 | 0.60 | 0.20 |
| Serine | ME700707 | 5.44 | 0.04 | 0.01 | 1.08 | 0.02 | 0.04 | 0.01 | 5.11 | NA | 0.04 | 0.02 | 0.17 | 0.02 |
| Threonine | ME700708 | 5.57 | 0.01 | 0.01 | 1.29 | 0.01 | 0.01 | 0.00 | 4.80 | NA | 0.01 | 0.00 | 0.28 | 0.01 |
| Histidine | ME700699 | 1.63 | 1.59 | 0.00 | 1.33 | 0.00 | 1.62 | 0.00 | 1.79 | NA | 1.36 | NA | 1.66 | 0.00 |
| Arginine | ME700692 | 2.00 | 1.62 | 0.01 | 1.59 | 0.00 | 1.62 | 0.00 | 1.88 | NA | 1.69 | 0.00 | 1.59 | 0.00 |
| Lysine | ME700702 | 1.99 | 1.80 | 0.08 | 1.67 | 0.05 | 1.90 | 0.08 | 2.17 | 0.02 | 1.62 | 0.06 | 1.48 | 0.07 |
| Methionine | ME700703 | 2.17 | 1.83 | 0.06 | 1.63 | 0.04 | 1.78 | 0.06 | 2.03 | 0.04 | 1.44 | 0.05 | 1.82 | 0.05 |
| Tyrosine | ME700710 | 2.13 | 1.75 | 0.07 | 1.66 | 0.05 | 1.60 | 0.07 | 2.15 | 0.02 | 1.53 | 0.06 | 1.84 | 0.06 |
| Leucine | ME700701 | 2.02 | 1.84 | 0.04 | 1.83 | 0.02 | 1.83 | 0.05 | 2.01 | 0.03 | 1.52 | 0.05 | 1.74 | 0.03 |
| Tryptophan | ME700709 | 1.99 | 1.82 | 0.01 | 1.71 | 0.01 | 1.78 | 0.01 | 1.97 | 0.01 | 1.56 | 0.01 | 1.77 | 0.01 |
| Phenylalanine | ME700705 | 1.88 | 1.91 | 0.02 | 1.66 | 0.02 | 1.76 | 0.02 | 2.11 | 0.02 | 1.65 | 0.02 | 1.89 | 0.02 |
| Isoleucine | ME700700 | 1.96 | 1.93 | 0.03 | 1.56 | 0.02 | 1.89 | 0.04 | 1.95 | 0.02 | 1.74 | 0.05 | 1.80 | 0.03 |
| Valine | ME700711 | 1.90 | 1.88 | 0.04 | 1.58 | 0.02 | 1.89 | 0.05 | 2.01 | 0.02 | 1.68 | 0.06 | 1.84 | 0.03 |
| Alanine | ME700691 | 2.43 | 1.21 | 0.29 | 1.40 | 0.19 | 1.25 | 0.33 | 2.70 | 0.03 | 1.22 | 0.25 | 1.23 | 0.23 |
| Glycine | ME700698 | 2.10 | 1.12 | 0.03 | 1.06 | 0.01 | 1.23 | 0.01 | 1.68 | NA | 1.42 | NA | 1.01 | 0.02 |
| Citrulline | ME700695 | 0.43 | 0.85 | 1.01 | 0.48 | 1.88 | 0.69 | 0.76 | 0.66 | 3.38 | 0.59 | 0.78 | 0.73 | 0.75 |
| Glutamine | ME700697 | 0.11 | 0.08 | 3.29 | 0.05 | 0.61 | 0.09 | 3.11 | 0.14 | 0.96 | 0.09 | 2.12 | 0.05 | 1.68 |
| Glutamic Acid | ME700696 | 1.42 | 0.44 | 2.08 | 0.54 | 0.82 | 0.24 | 2.09 | 1.42 | 0.35 | 0.14 | 1.13 | 0.44 | 1.89 |
| Aspartic Acid | ME700694 | 1.19 | 0.55 | 1.36 | 0.73 | 1.79 | 0.39 | 1.72 | 1.28 | 0.40 | 0.36 | 1.31 | 0.59 | 1.33 |
| Ornithine | ME700704 | 0.66 | 0.68 | 2.28 | 0.45 | 1.32 | 0.57 | 1.66 | 0.75 | 0.25 | 0.37 | 1.31 | 0.82 | 1.89 |
| Proline | ME700706 | 1.02 | 0.83 | 1.51 | 0.72 | 1.03 | 0.79 | 1.33 | 1.03 | 0.33 | 0.67 | 1.08 | 0.81 | 1.85 |
Factors:
| F1 | Strain:N/A | Sample type:Media Only |
| F2 | Strain:nos complement | Sample type:Supernatant |
| F3 | Strain:nos complement | Sample type:Whole Cells |
| F4 | Strain:nos mutant | Sample type:Supernatant |
| F5 | Strain:nos mutant | Sample type:Whole Cells |
| F6 | Strain:nos SrrAB mutant nos complement | Sample type:Supernatant |
| F7 | Strain:nos SrrAB mutant nos complement | Sample type:Whole Cells |
| F8 | Strain:nos SrrAB mutant | Sample type:Supernatant |
| F9 | Strain:nos SrrAB mutant | Sample type:Whole Cells |
| F10 | Strain:SrrAB mutant | Sample type:Supernatant |
| F11 | Strain:SrrAB mutant | Sample type:Whole Cells |
| F12 | Strain:Wildtype | Sample type:Supernatant |
| F13 | Strain:Wildtype | Sample type:Whole Cells |
