Clustering data with hclust algorithm for (Study ST002127)
(Analysis AN003480)| Metabolite | Structure | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 |
|---|---|---|---|---|---|---|---|---|---|
| [13C]Alpha_Ketoglutarate | ME516749 | NA | 5.53 | 0.10 | 0.08 | NA | 0.06 | 0.14 | 0.09 |
| [13C]Malate | ME516752 | NA | 0.68 | 1.10 | 0.94 | NA | 0.89 | 1.29 | 1.09 |
| [13C]Citrate | ME516750 | NA | 0.60 | 1.17 | 0.98 | NA | 0.71 | 1.38 | 1.17 |
| [13C]Fumatate | ME516751 | NA | 0.64 | 1.30 | 0.99 | NA | 0.56 | 1.41 | 1.11 |
| [13C]Succinate | ME516753 | NA | 0.68 | 1.23 | 0.98 | NA | 0.62 | 1.38 | 1.10 |
| [13C, 15N]Glycine, 2TBDMS | ME516737 | 0.02 | 1.73 | 0.82 | 1.29 | 0.02 | 1.80 | 0.95 | 1.38 |
| [13C, 15N]Alanine, 2TBDMS | ME516734 | 0.23 | 2.26 | 0.62 | 1.03 | 0.22 | 2.22 | 0.51 | 0.91 |
| [13C, 15N]Proline, 2TBDMS | ME516744 | 0.23 | 2.30 | 0.61 | 1.12 | 0.22 | 2.17 | 0.40 | 0.95 |
| [13C, 15N]Aspartic acid, 3TBDMS | ME516735 | 0.19 | 1.88 | 0.80 | 1.21 | 0.19 | 1.88 | 0.71 | 1.14 |
| [13C, 15N]Isoleucine, 2TBDMS | ME516739 | 0.17 | 1.74 | 0.75 | 1.24 | 0.20 | 1.97 | 0.65 | 1.27 |
| [13C, 15N]Phenylalanine, 2TBDMS | ME516743 | 0.21 | 2.06 | 0.93 | 1.08 | 0.18 | 1.85 | 0.71 | 0.98 |
| [13C, 15N]Threonine, 3TBDMS | ME516746 | 0.21 | 2.10 | 0.84 | 1.23 | 0.18 | 1.84 | 0.66 | 0.93 |
| [13C, 15N]Serine, 3TBDMS | ME516745 | 0.20 | 1.95 | 0.57 | 1.30 | 0.21 | 2.13 | 0.49 | 1.15 |
| [13C, 15N]Glutamic acid, 3TBDMS | ME516736 | 0.20 | 2.03 | 0.75 | 1.30 | 0.20 | 2.00 | 0.57 | 0.96 |
| [13C, 15N]Lysine, 3TBDMS | ME516741 | 0.19 | 1.94 | 0.80 | 1.10 | 0.20 | 2.04 | 0.63 | 1.10 |
| [13C, 15N]Methionine, 2TBDMS | ME516742 | 0.20 | 2.01 | 0.68 | 1.14 | 0.20 | 2.04 | 0.58 | 1.14 |
| [13C, 15N]Valine, 2TBDMS | ME516748 | 0.20 | 2.01 | 0.75 | 1.19 | 0.20 | 2.03 | 0.57 | 1.05 |
| [13C, 15N]Tyrosine, 3TBDMS | ME516747 | 0.22 | 2.17 | 0.71 | 1.11 | 0.20 | 2.05 | 0.56 | 0.98 |
| [13C, 15N]Histidine, 3TBDMS | ME516738 | 0.23 | 2.25 | 0.64 | 1.24 | 0.20 | 2.00 | 0.47 | 0.98 |
| [13C, 15N]Leucine, 2TBDMS | ME516740 | 0.21 | 2.13 | 0.64 | 1.33 | 0.20 | 2.00 | 0.48 | 1.01 |
Factors:
| F1 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) |
| F2 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F3 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F4 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F5 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) |
| F6 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F7 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F8 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
