Data for (Study ST002126)
(Analysis AN003479)Values for each metabolite have been scaled by dividing by the mean across all factors Run Hierarchial cluster analysis on this study | Run Heatmap cluster analysis on this study| Metabolite | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 |
|---|---|---|---|---|---|---|---|---|---|---|
| [13C, 15N]Alanine, 2TBDMS | 0.0659 | 2.7105 | 0.8177 | 1.4151 | 0.0599 | NA | 0.0619 | 3.0149 | 0.1564 | 0.6979 |
| [13C, 15N]Aspartic acid, 3TBDMS | 0.4016 | 2.2764 | 1.1179 | 1.4644 | 0.2810 | NA | 0.2923 | 2.0871 | 0.3365 | 0.7429 |
| [13C, 15N]Glutamic acid, 3TBDMS | 0.3182 | 2.3391 | 0.8957 | 1.5890 | 0.1440 | NA | 0.2906 | 2.4688 | 0.1636 | 0.7909 |
| [13C, 15N]Glycine, 2TBDMS | 0.0251 | 2.2996 | 1.2377 | 1.5327 | 0.0185 | NA | 0.0232 | 2.2741 | 0.5848 | 1.0044 |
| [13C, 15N]Histidine, 3TBDMS | 0.2927 | 2.6520 | 0.7297 | 1.5129 | 0.2385 | NA | 0.2670 | 2.6698 | 0.1495 | 0.4879 |
| [13C, 15N]Isoleucine, 2TBDMS | 0.1498 | 2.4510 | 1.0828 | 1.7213 | 0.1171 | NA | 0.1339 | 2.5157 | 0.2110 | 0.6176 |
| [13C, 15N]Leucine, 2TBDMS | 0.1777 | 2.3707 | 0.7771 | 1.5458 | 0.1199 | NA | 0.1656 | 2.8424 | 0.2012 | 0.7996 |
| [13C, 15N]Lysine, 3TBDMS | 0.1711 | 2.6423 | 1.0180 | 1.6754 | 0.1194 | NA | 0.2110 | 2.4695 | 0.0892 | 0.6041 |
| [13C, 15N]Methionine, 2TBDMS | 0.2975 | 2.6715 | 0.8204 | 1.4490 | 0.2298 | NA | 0.2585 | 2.4222 | 0.1429 | 0.7082 |
| [13C, 15N]Phenylalanine, 2TBDMS | 0.1484 | 2.4249 | 1.1410 | 1.3947 | 0.1283 | NA | 0.1603 | 2.6815 | 0.2774 | 0.6434 |
| [13C, 15N]Proline, 2TBDMS | 0.2361 | 2.6904 | 0.7684 | 1.2868 | 0.1466 | NA | 0.2719 | 2.7193 | 0.1932 | 0.6874 |
| [13C, 15N]Serine, 3TBDMS | 0.1143 | 2.6750 | 0.7219 | 1.7951 | 0.0707 | NA | 0.1467 | 2.6484 | 0.1391 | 0.6888 |
| [13C, 15N]Threonine, 3TBDMS | 0.0699 | 2.5663 | 1.0382 | 1.4871 | 0.0512 | NA | 0.0819 | 2.5601 | 0.2936 | 0.8517 |
| [13C, 15N]Tyrosine, 3TBDMS | 0.3406 | 2.7678 | 0.6814 | 1.2579 | 0.2076 | NA | 0.3426 | 2.6084 | 0.1588 | 0.6351 |
| [13C, 15N]Valine, 2TBDMS | 0.0236 | 2.5190 | 1.0574 | 1.6218 | 0.0157 | NA | 0.0237 | 2.6516 | 0.2951 | 0.7921 |
| [13C]Alpha_Ketoglutarate | 4.8027 | 0.0038 | 0.0057 | 0.0050 | 3.1548 | NA | NA | 0.0052 | 0.0150 | 0.0079 |
| [13C]Citrate | 4.9662 | 0.0265 | 0.0337 | 0.0288 | 2.7394 | NA | NA | 0.0481 | 0.0887 | 0.0687 |
| [13C]Fumatate | 4.7712 | 0.0072 | 0.0091 | 0.0090 | 3.1468 | NA | NA | 0.0110 | 0.0264 | 0.0192 |
| [13C]Malate | 5.4647 | 0.0028 | 0.0034 | 0.0031 | 2.5018 | NA | NA | 0.0064 | 0.0104 | 0.0075 |
| [13C]Succinate | 4.8396 | 0.0025 | 0.0039 | 0.0034 | 3.1324 | NA | NA | 0.0036 | 0.0090 | 0.0056 |
| Alanine, 2TBDMS | 2.1304 | NA | NA | NA | 0.5970 | 0.3743 | 0.8983 | NA | NA | NA |
| Alpha_Ketoglutarate | 1.4227 | NA | NA | NA | 0.9345 | 0.7614 | 0.8814 | NA | NA | NA |
| Aspartic acid, 3TBDMS | 2.3212 | NA | NA | NA | 0.7350 | 0.3416 | 0.6021 | NA | NA | NA |
| Citrate | 2.0731 | NA | NA | NA | 1.1435 | 0.2092 | 0.5741 | NA | NA | NA |
| Fumatate | 1.3959 | NA | NA | NA | 0.9206 | 0.7756 | 0.9079 | NA | NA | NA |
| Glutamic acid, 3TBDMS | 2.4073 | NA | NA | NA | 0.6771 | 0.2434 | 0.6722 | NA | NA | NA |
| Glutamine, 3TBDMS | 2.3806 | NA | NA | NA | 0.5719 | 0.3343 | 0.7132 | NA | NA | NA |
| Glycine, 2TBDMS | 2.1912 | NA | NA | NA | 0.9067 | 0.3707 | 0.5314 | NA | NA | NA |
| Histidine, 3TBDMS | 2.8228 | NA | NA | NA | 0.5045 | 0.2682 | 0.4045 | NA | NA | NA |
| Hydroxyproline, (E)-, 3TBDMS | 2.3651 | NA | NA | NA | 0.5905 | 0.3317 | 0.7126 | NA | NA | NA |
| Isoleucine, 2TBDMS | 2.3110 | NA | NA | NA | 0.7163 | 0.3683 | 0.6044 | NA | NA | NA |
| L-Cysteine, 3TBDMS | 3.1116 | NA | NA | NA | 0.7835 | 0.0338 | 0.0711 | NA | NA | NA |
| Leucine, 2TBDMS | 2.4369 | NA | NA | NA | 0.7407 | 0.2965 | 0.5259 | NA | NA | NA |
| Lysine, 3TBDMS | 2.4109 | NA | NA | NA | 0.5912 | 0.3571 | 0.6407 | NA | NA | NA |
| Malate | 1.7520 | NA | NA | NA | 0.8021 | 0.6044 | 0.8415 | NA | NA | NA |
| Methionine, 2TBDMS | 2.6241 | NA | NA | NA | 0.6570 | 0.2164 | 0.5025 | NA | NA | NA |
| Phenylalanine, 2TBDMS | 1.9942 | NA | NA | NA | 0.7042 | 0.4683 | 0.8332 | NA | NA | NA |
| Proline, 2TBDMS | 2.6549 | NA | NA | NA | 0.4524 | 0.2211 | 0.6716 | NA | NA | NA |
| Serine, 3TBDMS | 2.1881 | NA | NA | NA | 0.8388 | 0.3053 | 0.6678 | NA | NA | NA |
| Succinate | 1.4598 | NA | NA | NA | 0.9449 | 0.7544 | 0.8410 | NA | NA | NA |
| Threonine, 3TBDMS | 2.1946 | NA | NA | NA | 0.7437 | 0.3694 | 0.6923 | NA | NA | NA |
| Tyrosine, 3TBDMS | 2.3329 | NA | NA | NA | 0.6456 | 0.3671 | 0.6544 | NA | NA | NA |
| Valine, 2TBDMS | 2.4518 | NA | NA | NA | 0.6500 | 0.2922 | 0.6060 | NA | NA | NA |
Factors:
| F1 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| F2 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F3 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F4 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F5 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| F6 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:HSC Basal or Ctrl, NR-treatment 8 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig5J). |
| F7 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | Batch:1c | Note:[13C, 15N] AAs were used for AA uptake (Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig5J). |
| F8 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F9 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
| F10 | Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 8 weeks, 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1d | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). |
