Statistics Toolbox for Study: ST000240

Title: Global LC-MS of Ozone Stress in Maize: GLCMS

Run analyses on data in Study ST000240 Dataset: HESI positive ion mode

Metabolite classes (all analyses combined)

• Pie chart of metabolite super classes
• Pie chart of metabolite main classes
• Pie chart of metabolite sub classes

Normalization and averaging

• Show Metabolite averages per experimental factor
• Perform normalization on data
• Create Relative log abundance plots

Univariate analysis

• Perform multi-condition dot plot analysis
• Perform Volcano plot analysis
• Perform ANOVA analysis

MetENP: Metabolite enrichment and species-specific pathway annotation

• MetENPWeb analysis
• MetENP R package
• MetENP tutorial

Clustering and correlation

• Perform hierarchial or heatmap cluster analysis
• Perform Clustered correlation analysis
• Perform Network analysis on correlated metabolites

Multivariate analysis

• Perform Principal component analysis
• Perform Linear discriminant analysis
• Perform Partial least-squares discriminant analysis (PLS-DA)

Classification and feature analysis

• Perform OPLS-DA and VIP projection
• Random Forest and VIP projection

Mapping metabolites to human biochemical pathways

• Not applicable (non-mammalian)