Metabolomics Workbench : NIH Data Repository

Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1	Experimental factor	Group2
	2 ()
	Categorization:Amhara \| Country:Ethiopia \| Ethnicity:Amhara \| Occupation:Agriculturalist \| Sex:F (11)
	Categorization:Amhara \| Country:Ethiopia \| Ethnicity:Amhara \| Occupation:Agriculturalist \| Sex:M (18)
	Categorization:Dizi \| Country:Ethiopia \| Ethnicity:Dizi \| Occupation:Agropastoralist \| Sex:F (18)
	Categorization:Dizi \| Country:Ethiopia \| Ethnicity:Dizi \| Occupation:Agropastoralist \| Sex:M (11)
	Categorization:Hadza_settled \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:F (17)
	Categorization:Hadza_settled \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:M (13)
	Categorization:Hadza_traditional \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:F (10)
	Categorization:Hadza_traditional \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:M (19)
	Categorization:Hadza_traditional \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:nan (1)
	Categorization:Hamer \| Country:Ethiopia \| Ethnicity:Hamer \| Occupation:Pastoralist \| Sex:F (17)
	Categorization:Hamer \| Country:Ethiopia \| Ethnicity:Hamer \| Occupation:Pastoralist \| Sex:M (13)
	Categorization:Herero \| Country:Botswana \| Ethnicity:Herero/Na \| Occupation:Pastoralist \| Sex:F (1)
	Categorization:Herero \| Country:Botswana \| Ethnicity:Herero \| Occupation:Pastoralist \| Sex:F (24)
	Categorization:Herero \| Country:Botswana \| Ethnicity:Herero \| Occupation:Pastoralist \| Sex:M (5)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan \| Occupation:Hunter-Gatherer \| Sex:F (7)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan \| Occupation:Hunter-Gatherer \| Sex:M (4)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan/!Xoo \| Occupation:Hunter-Gatherer \| Sex:F (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan/!Xoo \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Ju\|'Hoan \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Naro \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau \| Occupation:Hunter-Gatherer \| Sex:F (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau \| Occupation:Hunter-Gatherer \| Sex:M (4)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Tsoa/Naro/Na \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Tsoa \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Tswana \| Occupation:Hunter-Gatherer \| Sex:F (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Naro/Kaukau/Nama/Na \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Naro/Kaukau \| Occupation:Hunter-Gatherer \| Sex:F (6)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Kgalagadi/Tswana \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Mbukushu \| Occupation:Agropastoralist \| Sex:F (5)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Mbukushu \| Occupation:Agropastoralist \| Sex:M (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana/Herero \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana/Na \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana/Na \| Occupation:Agropastoralist \| Sex:M (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana \| Occupation:Agropastoralist \| Sex:F (11)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana \| Occupation:Agropastoralist \| Sex:M (6)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Yeyi \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Yeyi \| Occupation:Agropastoralist \| Sex:M (2)
	Categorization:Mursi \| Country:Ethiopia \| Ethnicity:Mursi \| Occupation:Pastoralist \| Sex:F (7)
	Categorization:Mursi \| Country:Ethiopia \| Ethnicity:Mursi \| Occupation:Pastoralist \| Sex:M (23)
	Categorization:Naro \| Country:Botswana \| Ethnicity:Naro \| Occupation:Hunter-Gatherer \| Sex:F (21)
	Categorization:Naro \| Country:Botswana \| Ethnicity:Naro \| Occupation:Hunter-Gatherer \| Sex:M (9)
	Categorization:Qimant \| Country:Ethiopia \| Ethnicity:Qimant \| Occupation:Agropastoralist \| Sex:F (18)
	Categorization:Qimant \| Country:Ethiopia \| Ethnicity:Qimant \| Occupation:Agropastoralist \| Sex:M (12)
	Categorization:Sabue \| Country:Ethiopia \| Ethnicity:Sabue \| Occupation:Hunter-Gatherer \| Sex:F (11)
	Categorization:Sabue \| Country:Ethiopia \| Ethnicity:Sabue \| Occupation:Hunter-Gatherer \| Sex:M (19)
	Categorization:Sandawe \| Country:Tanzania \| Ethnicity:Sandawe \| Occupation:Hunter-Gatherer \| Sex:F (17)
	Categorization:Sandawe \| Country:Tanzania \| Ethnicity:Sandawe \| Occupation:Hunter-Gatherer \| Sex:M (13)
	Categorization:Weyto \| Country:Ethiopia \| Ethnicity:Weyto \| Occupation:Hunter-Gatherer \| Sex:F (16)
	Categorization:Weyto \| Country:Ethiopia \| Ethnicity:Weyto \| Occupation:Hunter-Gatherer \| Sex:M (14)
	Categorization:!Xoo \| Country:Botswana \| Ethnicity:!Xoo \| Occupation:Hunter-Gatherer \| Sex:F (20)
	Categorization:!Xoo \| Country:Botswana \| Ethnicity:!Xoo \| Occupation:Hunter-Gatherer \| Sex:M (9)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold must exceed the above threshold per analyte In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff:	Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:NEGATIVE	MS search m/z tolerance:
Ion adducts(choose at least one)
Negative mode: [M-H]- [M+Cl]- [M+HCOO]- [M+OAc]- [M-CH3]- [M-2H]2- [M-3H]3- [M-H-Ser]-

Group1	Experimental factor	Group2
	2 ()
	Categorization:Amhara \| Country:Ethiopia \| Ethnicity:Amhara \| Occupation:Agriculturalist \| Sex:F (11)
	Categorization:Amhara \| Country:Ethiopia \| Ethnicity:Amhara \| Occupation:Agriculturalist \| Sex:M (18)
	Categorization:Dizi \| Country:Ethiopia \| Ethnicity:Dizi \| Occupation:Agropastoralist \| Sex:F (18)
	Categorization:Dizi \| Country:Ethiopia \| Ethnicity:Dizi \| Occupation:Agropastoralist \| Sex:M (11)
	Categorization:Hadza_settled \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:F (17)
	Categorization:Hadza_settled \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:M (13)
	Categorization:Hadza_traditional \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:F (10)
	Categorization:Hadza_traditional \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:M (19)
	Categorization:Hadza_traditional \| Country:Tanzania \| Ethnicity:Hadza \| Occupation:Hunter-Gatherer \| Sex:nan (1)
	Categorization:Hamer \| Country:Ethiopia \| Ethnicity:Hamer \| Occupation:Pastoralist \| Sex:F (17)
	Categorization:Hamer \| Country:Ethiopia \| Ethnicity:Hamer \| Occupation:Pastoralist \| Sex:M (13)
	Categorization:Herero \| Country:Botswana \| Ethnicity:Herero/Na \| Occupation:Pastoralist \| Sex:F (1)
	Categorization:Herero \| Country:Botswana \| Ethnicity:Herero \| Occupation:Pastoralist \| Sex:F (24)
	Categorization:Herero \| Country:Botswana \| Ethnicity:Herero \| Occupation:Pastoralist \| Sex:M (5)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan \| Occupation:Hunter-Gatherer \| Sex:F (7)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan \| Occupation:Hunter-Gatherer \| Sex:M (4)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan/!Xoo \| Occupation:Hunter-Gatherer \| Sex:F (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Ju\|'Hoan/!Xoo \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Ju\|'Hoan \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Naro \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau \| Occupation:Hunter-Gatherer \| Sex:F (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau \| Occupation:Hunter-Gatherer \| Sex:M (4)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Tsoa/Naro/Na \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Tsoa \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Kaukau/Tswana \| Occupation:Hunter-Gatherer \| Sex:F (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Naro/Kaukau/Nama/Na \| Occupation:Hunter-Gatherer \| Sex:M (1)
	Categorization:Ju \| Country:Botswana \| Ethnicity:Naro/Kaukau \| Occupation:Hunter-Gatherer \| Sex:F (6)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Kgalagadi/Tswana \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Mbukushu \| Occupation:Agropastoralist \| Sex:F (5)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Mbukushu \| Occupation:Agropastoralist \| Sex:M (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana/Herero \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana/Na \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana/Na \| Occupation:Agropastoralist \| Sex:M (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana \| Occupation:Agropastoralist \| Sex:F (11)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Tswana \| Occupation:Agropastoralist \| Sex:M (6)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Yeyi \| Occupation:Agropastoralist \| Sex:F (1)
	Categorization:Mb_Ts_Ye \| Country:Botswana \| Ethnicity:Yeyi \| Occupation:Agropastoralist \| Sex:M (2)
	Categorization:Mursi \| Country:Ethiopia \| Ethnicity:Mursi \| Occupation:Pastoralist \| Sex:F (7)
	Categorization:Mursi \| Country:Ethiopia \| Ethnicity:Mursi \| Occupation:Pastoralist \| Sex:M (23)
	Categorization:Naro \| Country:Botswana \| Ethnicity:Naro \| Occupation:Hunter-Gatherer \| Sex:F (21)
	Categorization:Naro \| Country:Botswana \| Ethnicity:Naro \| Occupation:Hunter-Gatherer \| Sex:M (9)
	Categorization:Qimant \| Country:Ethiopia \| Ethnicity:Qimant \| Occupation:Agropastoralist \| Sex:F (18)
	Categorization:Qimant \| Country:Ethiopia \| Ethnicity:Qimant \| Occupation:Agropastoralist \| Sex:M (12)
	Categorization:Sabue \| Country:Ethiopia \| Ethnicity:Sabue \| Occupation:Hunter-Gatherer \| Sex:F (11)
	Categorization:Sabue \| Country:Ethiopia \| Ethnicity:Sabue \| Occupation:Hunter-Gatherer \| Sex:M (19)
	Categorization:Sandawe \| Country:Tanzania \| Ethnicity:Sandawe \| Occupation:Hunter-Gatherer \| Sex:F (17)
	Categorization:Sandawe \| Country:Tanzania \| Ethnicity:Sandawe \| Occupation:Hunter-Gatherer \| Sex:M (13)
	Categorization:Weyto \| Country:Ethiopia \| Ethnicity:Weyto \| Occupation:Hunter-Gatherer \| Sex:F (16)
	Categorization:Weyto \| Country:Ethiopia \| Ethnicity:Weyto \| Occupation:Hunter-Gatherer \| Sex:M (14)
	Categorization:!Xoo \| Country:Botswana \| Ethnicity:!Xoo \| Occupation:Hunter-Gatherer \| Sex:F (20)
	Categorization:!Xoo \| Country:Botswana \| Ethnicity:!Xoo \| Occupation:Hunter-Gatherer \| Sex:M (9)