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Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features
Group1
Experimental factor
Group2
2 ()
Parent:BC233_BC240_mix (11)
Parent:BC233(YPS606) (6)
Parent:BC240(UCD2120) (6)
Parent:F2_2A1 (3)
Parent:F2_2A10 (3)
Parent:F2_2A11 (4)
Parent:F2_2A12 (2)
Parent:F2_2A3 (2)
Parent:F2_2A4 (3)
Parent:F2_2A5 (3)
Parent:F2_2A6 (3)
Parent:F2_2A7 (4)
Parent:F2_2A8 (3)
Parent:F2_2A9 (3)
Parent:F2_2B1 (3)
Parent:F2_2B10 (3)
Parent:F2_2B11 (3)
Parent:F2_2B3 (3)
Parent:F2_2B4 (3)
Parent:F2_2B5 (3)
Parent:F2_2B6 (3)
Parent:F2_2B8 (3)
Parent:F2_2B9 (3)
Parent:F2_2C1 (3)
Parent:F2_2C11 (3)
Parent:F2_2C12 (3)
Parent:F2_2C2 (3)
Parent:F2_2C3 (3)
Parent:F2_2C4 (2)
Parent:F2_2C6 (3)
Parent:F2_2C7 (3)
Parent:F2_2C8 (3)
Parent:F2_2C9 (3)
Parent:F2_2D1 (3)
Parent:F2_2D10 (2)
Parent:F2_2D11 (3)
Parent:F2_2D12 (3)
Parent:F2_2D2 (2)
Parent:F2_2D_2 (1)
Parent:F2_2D4 (3)
Parent:F2_2D5 (3)
Parent:F2_2D6 (3)
Parent:F2_2D7 (3)
Parent:F2_2D8 (1)
Parent:F2_2D9 (3)
Parent:F2_2E10 (2)
Parent:F2_2E12 (3)
Parent:F2_2E2 (3)
Parent:F2_2E4 (3)
Parent:F2_2E5 (3)
Parent:F2_2E7 (3)
Parent:F2_2E8 (3)
Parent:F2_2E9 (3)
Parent:F2_2F11 (3)
Parent:F2_2F12 (3)
Parent:F2_2F2 (3)
Parent:F2_2F3 (3)
Parent:F2_2F5 (3)
Parent:F2_2F6 (3)
Parent:F2_2F7 (3)
Parent:F2_2F8 (3)
Parent:F2_2G1 (3)
Parent:F2_2G10 (4)
Parent:F2_2G11 (3)
Parent:F2_2G3 (3)
Parent:F2_2G4 (3)
Parent:F2_2G5 (3)
Parent:F2_2G6 (4)
Parent:F2_2G8 (2)
Parent:F2_2G9 (4)
Parent:F2_7A1 (3)
Parent:F2_7A10 (3)
Parent:F2_7A11 (4)
Parent:F2_7A12 (4)
Parent:F2_7A2 (3)
Parent:F2_7A3 (3)
Parent:F2_7A4 (3)
Parent:F2_7A5 (3)
Parent:F2_7A6 (3)
Parent:F2_7A8 (3)
Parent:F2_7A9 (3)
Parent:F2_7B10 (3)
Parent:F2_7B11 (3)
Parent:F2_7B3 (3)
Parent:F2_7B4 (3)
Parent:F2_7B5 (3)
Parent:F2_7B6 (3)
Parent:F2_7B8 (3)
Parent:F2_7B9 (3)
Parent:F2_7C1 (3)
Parent:F2_7C11 (3)
Parent:F2_7C12 (3)
Parent:F2_7C2 (3)
Parent:F2_7C3 (3)
Parent:F2_7C4 (3)
Parent:F2_7C6 (3)
Parent:F2_7C7 (3)
Parent:F2_7C8 (3)
Parent:F2_7C9 (3)
Parent:F2_7D1 (3)
Parent:F2_7D10 (2)
Parent:F2_7D11 (3)
Parent:F2_7D2 (3)
Parent:F2_7D4 (3)
Parent:F2_7D6 (3)
Parent:F2_7D7 (3)
Parent:F2_7D8 (1)
Parent:F2_7D9 (3)
Parent:F2_7E1 (3)
Parent:F2_7E10 (3)
Parent:F2_7E4 (3)
Parent:F2_7E7 (3)
Parent:F2_7E8 (3)
Parent:F2_7F1 (3)
Parent:F2_7F10 (3)
Parent:F2_7F11 (3)
Parent:F2_7F12 (3)
Parent:F2_7F2 (3)
Parent:F2_7F3 (3)
Parent:F2_7F5 (3)
Parent:F2_7F6 (4)
Parent:F2_7F7 (3)
Parent:F2_7F8 (3)
Parent:F2_7G1 (3)
Parent:F2_8A1 (3)
Parent:F2_8A10 (2)
Parent:F2_8A11 (2)
Parent:F2_8A12 (2)
Parent:F2_8A2 (2)
Parent:F2_8A3 (3)
Parent:F2_8A5 (2)
Parent:F2_8A6 (3)
Parent:F2_8A7 (3)
Parent:F2_8A9 (3)
Parent:F2_8B11 (3)
Parent:F2_8B5 (3)
Parent:F2_8B6 (3)
Parent:F2_8B9 (3)
Parent:F2_8C1 (3)
Parent:F2_8C112 (1)
Parent:F2_8C12 (2)
Parent:F2_8C4 (3)
Parent:F2_8C6 (3)
Parent:F2_8C7 (3)
Parent:F2_8C9 (3)
Parent:F2_8D11 (1)
Parent:F2_8D2 (3)
Parent:F2_8D5 (3)
Parent:F2_8D7 (3)
Parent:F2_8D8 (2)
Parent:F2_8E2 (1)
Parent:F2_8F1 (1)
Parent:F2_8F10 (2)
Parent:F2_8F11 (3)
Parent:F2_8F2 (1)
Parent:F2_8F5 (1)
Parent:F2_8G1 (3)
Parent:F2_8G5 (3)
Parent:F2_8G6 (1)
Parent:media only (6)
Parent:no cells/no media (7)
Filtering Parameters
Select threshold % for selection below:
100% (option 4 only)
95%
90%
85%
80%
75%
70%
65%
60%
55%
50%
45%
40%
35%
30%
20%
10%
0%
Option 1:
No filtering
Option 2:
Filter by Sum
(Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3:
Filter by Mean
(Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4:
Filter by Median
(Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5:
Filter by Sample threshold
At least one of the 2 selected groups
Both groups
must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6:
Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
None
Sample means
Sample sums
Volcano Plot Parameters
P-value cutoff:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Fold-change cutoff:
1.1
1.2
1.25
1.33
1.5
2
3
4
5
MS Search Parameters
Search Refmet database
MS Ion polarity:NEGATIVE
MS search m/z tolerance:
0.1
0.05
0.02
0.01
0.005
0.002
0.001
0.0005
0.0002
Feature order:
m/z_rt
rt_m/z
Ion adducts(choose at least one)
Negative mode:
[M-H]-
[M+Cl]-
[M+HCOO]-
[M+OAc]-
[M-CH3]-
[M-2H]2-
[M-3H]3-
[M-H-Ser]-
Metabolomics Workbench
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