Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Detail:Non-labeled Ophiorrhiza pumila root (1)
Detail:Non-labeled Oryza sativa leaf&stem (1)
Detail:Non-labeled Oryza sativa root (1)
Detail:Non-labeled Solanum lycopersicum fruit green (1)
Detail:Non-labeled Solanum lycopersicum leaf&stem (1)
Detail:Non-labeled Solanum lycopersicum ripe (1)
Detail:Non-labeled Zea mays leaf (1)
Detail:Non-labeled Zea mays root (1)
Detail:Non-labeled Zea mays seed (1)
Detail:Non-labeled Zea mays stem (1)
Detail:Non-labelled Glycine max leaf&stem (1)
Detail:Non-labelled Glycine max root (1)
Detail:Non-labelled Glycyrrhiza glabra leaf&stem (1)
Detail:Non-labelled Glycyrrhiza glabra root (1)
Detail:Non-labelled Glycyrrhiza uralensis leaf&stem (1)
Detail:Non-labelled Glycyrrhiza uralensis root (1)
Detail:Non-labelled Medicago truncatula flower (1)
Detail:Non-labelled Medicago truncatula leaf&stem (1)
Detail:Non-labelled Medicago truncatula ripe pod (1)
Detail:Non-labelled Medicago truncatula root (1)
Detail:Non-labelled Medicago truncatula seed (1)
Detail:Non-labelled reference Arabidopsis thaliana leaf&stem (1)
Detail:Non-labelled reference Arabidopsis thaliana root (1)
Detail:Non-labelled Solanum tuberosum leaf&stem (1)
Detail:Non-labelled Solanum tuberosum root (1)
Detail:Non-labelled Solanum tuberosum tuber (1)
Detail:Non labeled Allium cepa bulb (1)
Detail:Non labeled Allium cepa leaf (1)
Detail:Non labeled Allium cepa root (1)
Detail:Non labeled Nicotiana tabacum leaf&stem (1)
Detail:Non labeled Nicotiana tabacum root (1)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Positive mode:
 [M+H]+ [M+H-H2O]+ [M+Na]+ [M+NH4]+ [M+K]+ [M+2H]2+ [M+2Na]2+ [M+2Na-H]+
[M+H-EtnP]+ [M+H-SerP]+
  logo