Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Species:Alexandrium tamarense | Strain:1771 | Broad taxon:Dinoflagellate (3)
Species:Amphidinium carterae | Strain:1314 | Broad taxon:Dinoflagellate (3)
Species:Crocosphaera watsonii | Strain:8501 | Broad taxon:Cyanobacteria (3)
Species:Cyclotella meneghiniana | Strain:338 | Broad taxon:Diatom (3)
Species:Emiliania huxleyi | Strain:2090 | Broad taxon:Haptophyte (3)
Species:Emiliania huxleyi | Strain:371 | Broad taxon:Haptophyte (3)
Species:Heterocapsa triquetra | Strain:449 | Broad taxon:Dinoflagellate (3)
Species:Lingulodinium polyedra | Strain:2021 | Broad taxon:Dinoflagellate (3)
Species:Micromonas pusilla | Strain:1545 | Broad taxon:Prasinophyte (3)
Species:Navicula pelliculosa | Strain:543 | Broad taxon:Diatom (3)
Species:Nitrosopumilus maritimus | Strain:SCM1 | Broad taxon:Archaea (3)
Species:Ostreococcus lucimarinus | Strain:3430 | Broad taxon:Prasinophyte (3)
Species:Phaeodactylum tricornutum | Strain:2561 | Broad taxon:Diatom (2)
Species:Prochlorococcus marinus | Strain:1314 | Broad taxon:Cyanobacteria (3)
Species:Prochlorococcus marinus | Strain:AS9601 | Broad taxon:Cyanobacteria (3)
Species:Prochlorococcus marinus | Strain:MED4 | Broad taxon:Cyanobacteria (3)
Species:Prochlorococcus marinus | Strain:NATL2A | Broad taxon:Cyanobacteria (3)
Species:Pseudo-nitzschia pungens | Strain:Pc55x | Broad taxon:Diatom (3)
Species:Synechococcus sp. | Strain:7803 | Broad taxon:Cyanobacteria (2)
Species:Synechococcus sp. | Strain:8102 | Broad taxon:Cyanobacteria (2)
Species:Thalassiosira oceanica | Strain:1005 | Broad taxon:Diatom (3)
Species:Thalassiosira pseudonana | Strain:1335 | Broad taxon:Diatom (3)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Positive mode:
 [M+H]+ [M+H-H2O]+ [M+Na]+ [M+NH4]+ [M+K]+ [M+2H]2+ [M+2Na]2+ [M+2Na-H]+
[M+H-EtnP]+ [M+H-SerP]+
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