Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Fiber:Arabinoxylan | Timepoint:10 | Sex:F (11)
Fiber:Arabinoxylan | Timepoint:10 | Sex:M (8)
Fiber:Arabinoxylan | Timepoint:20 | Sex:F (10)
Fiber:Arabinoxylan | Timepoint:20 | Sex:M (8)
Fiber:Arabinoxylan | Timepoint:30 | Sex:F (10)
Fiber:Arabinoxylan | Timepoint:30 | Sex:M (8)
Fiber:Arabinoxylan | Timepoint:Baseline | Sex:F (10)
Fiber:Arabinoxylan | Timepoint:Baseline | Sex:M (9)
Fiber:Arabinoxylan | Timepoint:Month 1 | Sex:M (2)
Fiber:Arabinoxylan | Timepoint:Month 2 | Sex:M (2)
Fiber:Arabinoxylan | Timepoint:Month 3 | Sex:M (2)
Fiber:Arabinoxylan | Timepoint:Washout D10 | Sex:F (10)
Fiber:Arabinoxylan | Timepoint:Washout D10 | Sex:M (7)
Fiber:Arabinoxylan | Timepoint:Washout D3 | Sex:F (10)
Fiber:Arabinoxylan | Timepoint:Washout D3 | Sex:M (8)
Fiber:Arabinoxylan | Timepoint:Washout D30 | Sex:M (1)
Fiber:Arabinoxylan | Timepoint:Washout Week 3 | Sex:M (1)
Fiber:Arabinoxylan | Timepoint:Week 2 | Sex:M (1)
Fiber:Arabinoxylan | Timepoint:Week2 2 | Sex:M (1)
Fiber:Inulin | Timepoint:- | Sex:M (1)
Fiber:Inulin | Timepoint:10 | Sex:F (10)
Fiber:Inulin | Timepoint:10 | Sex:M (10)
Fiber:Inulin | Timepoint:20 | Sex:F (10)
Fiber:Inulin | Timepoint:20 | Sex:M (10)
Fiber:Inulin | Timepoint:30 | Sex:F (10)
Fiber:Inulin | Timepoint:30 | Sex:M (10)
Fiber:Inulin | Timepoint:Baseline | Sex:F (10)
Fiber:Inulin | Timepoint:Baseline | Sex:M (9)
Fiber:Inulin | Timepoint:End Washout | Sex:M (1)
Fiber:Inulin | Timepoint:Washout D10 | Sex:F (9)
Fiber:Inulin | Timepoint:Washout D10 | Sex:M (10)
Fiber:Inulin | Timepoint:Washout D3 | Sex:F (10)
Fiber:Inulin | Timepoint:Washout D3 | Sex:M (10)
Fiber:Inulin | Timepoint:Washout Week 3 | Sex:M (2)
Fiber:Mix | Timepoint:10 | Sex:F (9)
Fiber:Mix | Timepoint:10 | Sex:M (7)
Fiber:Mix | Timepoint:20 | Sex:F (9)
Fiber:Mix | Timepoint:20 | Sex:M (7)
Fiber:Mix | Timepoint:30 | Sex:F (8)
Fiber:Mix | Timepoint:30 | Sex:M (6)
Fiber:Mix | Timepoint:? | Sex:F (1)
Fiber:Mix | Timepoint:Baseline | Sex:F (8)
Fiber:Mix | Timepoint:Baseline | Sex:M (7)
Fiber:Mix | Timepoint:Washout D10 | Sex:F (9)
Fiber:Mix | Timepoint:Washout D10 | Sex:M (7)
Fiber:Mix | Timepoint:Washout D3 | Sex:F (8)
Fiber:Mix | Timepoint:Washout D3 | Sex:M (7)
Fiber:Mix | Timepoint:Washout Week 3 | Sex:M (1)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Positive mode:
 [M+H]+ [M+H-H2O]+ [M+Na]+ [M+NH4]+ [M+K]+ [M+2H]2+ [M+2Na]2+ [M+2Na-H]+
[M+H-EtnP]+ [M+H-SerP]+
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