Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Donor:1 | Treatment:Aronia | Time:0 (3)
Donor:1 | Treatment:Aronia | Time:2 (3)
Donor:1 | Treatment:Aronia | Time:4 (3)
Donor:1 | Treatment:Aronia | Time:6 (3)
Donor:1 | Treatment:Aronia | Time:8 (3)
Donor:1 | Treatment:Control | Time:0 (3)
Donor:1 | Treatment:Control | Time:2 (3)
Donor:1 | Treatment:Control | Time:4 (3)
Donor:1 | Treatment:Control | Time:6 (3)
Donor:1 | Treatment:Control | Time:8 (3)
Donor:7 | Treatment:Aronia | Time:0 (5)
Donor:7 | Treatment:Aronia | Time:2 (4)
Donor:7 | Treatment:Aronia | Time:4 (4)
Donor:7 | Treatment:Aronia | Time:6 (4)
Donor:7 | Treatment:Aronia | Time:8 (4)
Donor:7 | Treatment:Control | Time:0 (3)
Donor:7 | Treatment:Control | Time:2 (3)
Donor:7 | Treatment:Control | Time:4 (3)
Donor:7 | Treatment:Control | Time:6 (3)
Donor:7 | Treatment:Control | Time:8 (3)
Donor:Blank | Treatment:Blank | Time:Blank (1)
Donor:Extraction Blank | Treatment:Extraction Blank | Time:Extraction Blank (2)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Positive mode:
 [M+H]+ [M+H-H2O]+ [M+Na]+ [M+NH4]+ [M+K]+ [M+2H]2+ [M+2Na]2+ [M+2Na-H]+
[M+H-EtnP]+ [M+H-SerP]+
  logo