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MB Sample ID: SA001064
Local Sample ID: | S00009473 |
Subject ID: | SU000033 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Low Capacity Running | High Capacity Running |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000033 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Low Capacity Running | High Capacity Running |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S00009473 | SA001064 | FL000393 | Low | Rat running capacity |
S00009473 | SA001064 | FL000393 | Ad lib | Rat feeding regimen |
Collection:
Collection ID: | CO000017 |
Collection Summary: | - |
Sample Type: | Serum |
Volumeoramount Collected: | 50 ul |
Treatment:
Treatment ID: | TR000035 |
Treatment Summary: | ad lib feeding | Calorie restricted feeding |
Sample Preparation:
Sampleprep ID: | SP000030 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | EX00166-Sample_preparation_protocol.pdf |
Combined analysis:
Analysis ID | AN000034 | AN000035 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1200 | Agilent 1200 |
Column | Waters Acquity HSS T3 reversed-phase | Waters Acquity HSS T3 reversed-phase |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH000017 |
Chromatography Summary: | Positive ion detection |
Methods Filename: | EX00166-LCMS-method.pdf |
Instrument Name: | Agilent 1200 |
Column Name: | Waters Acquity HSS T3 reversed-phase |
Column Temperature: | 40C |
Flow Gradient: | 0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B. |
Flow Rate: | 0.35 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000018 |
Chromatography Summary: | Negative ion detection |
Methods Filename: | EX00166-LCMS-method.pdf |
Instrument Name: | Agilent 1200 |
Column Name: | Waters Acquity HSS T3 reversed-phase |
Column Temperature: | 40C |
Flow Gradient: | 0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B. |
Flow Rate: | 0.35 mL/min |
Solvent A: | 100% water; 0.1% ammonium bicarbonate |
Solvent B: | 100% methanol; 0.1% ammonium bicarbonate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000033 |
Analysis ID: | AN000034 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Reference masses were 99.09 and 922.009 m/z. |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500V |
Ion Source Temperature: | 350 C |
Acquisition Parameters File: | EX00166-LCMS-method.pdf |
Processing Parameters File: | EX00166-Data_analysis-method.pdf |
MS ID: | MS000034 |
Analysis ID: | AN000035 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Reference masses 62.0009 and 981.9956 m/z. |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 3500V |
Ion Source Temperature: | 350 C |
Acquisition Parameters File: | EX00166-LCMS-method.pdf |
Processing Parameters File: | EX00166-Data_analysis-method.pdf |