Return to study ST000017 main page
MB Sample ID: SA001064
| Local Sample ID: | S00009473 |
| Subject ID: | SU000033 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Low Capacity Running | High Capacity Running |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000033 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Low Capacity Running | High Capacity Running |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S00009473 | SA001064 | FL000393 | Low | Rat running capacity |
| S00009473 | SA001064 | FL000393 | Ad lib | Rat feeding regimen |
Collection:
| Collection ID: | CO000017 |
| Collection Summary: | - |
| Sample Type: | Serum |
| Volumeoramount Collected: | 50 ul |
Treatment:
| Treatment ID: | TR000035 |
| Treatment Summary: | ad lib feeding | Calorie restricted feeding |
Sample Preparation:
| Sampleprep ID: | SP000030 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | EX00166-Sample_preparation_protocol.pdf |
Combined analysis:
| Analysis ID | AN000034 | AN000035 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1200 | Agilent 1200 |
| Column | Waters Acquity HSS T3 reversed-phase | Waters Acquity HSS T3 reversed-phase |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000017 |
| Chromatography Summary: | Positive ion detection |
| Methods Filename: | EX00166-LCMS-method.pdf |
| Instrument Name: | Agilent 1200 |
| Column Name: | Waters Acquity HSS T3 reversed-phase |
| Column Temperature: | 40C |
| Flow Gradient: | 0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B. |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000018 |
| Chromatography Summary: | Negative ion detection |
| Methods Filename: | EX00166-LCMS-method.pdf |
| Instrument Name: | Agilent 1200 |
| Column Name: | Waters Acquity HSS T3 reversed-phase |
| Column Temperature: | 40C |
| Flow Gradient: | 0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B. |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% ammonium bicarbonate |
| Solvent B: | 100% methanol; 0.1% ammonium bicarbonate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000033 |
| Analysis ID: | AN000034 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Reference masses were 99.09 and 922.009 m/z. |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3500V |
| Ion Source Temperature: | 350 C |
| Acquisition Parameters File: | EX00166-LCMS-method.pdf |
| Processing Parameters File: | EX00166-Data_analysis-method.pdf |
| MS ID: | MS000034 |
| Analysis ID: | AN000035 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Reference masses 62.0009 and 981.9956 m/z. |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3500V |
| Ion Source Temperature: | 350 C |
| Acquisition Parameters File: | EX00166-LCMS-method.pdf |
| Processing Parameters File: | EX00166-Data_analysis-method.pdf |
