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MB Sample ID: SA010809

Local Sample ID:Control serum_Ahr+
Subject ID:SU000245
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J; Wild Type and Ahr-/-
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

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Subject:

Subject ID:SU000245
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J; Wild Type and Ahr-/-
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Control serum_Ahr+SA010809FL002576Malegender
Control serum_Ahr+SA010809FL002576Ahr +/+genotype
Control serum_Ahr+SA010809FL002576VehicleTreatment

Collection:

Collection ID:CO000233
Collection Summary:-
Sample Type:Feces, liver, cecal contents, intestine and blood (only liver and blood tested with NRM)

Treatment:

Treatment ID:TR000253
Treatment Summary:Wild type and AHR knock out mice (12 wild types and 10 AHR knock out) were treated with TCDF for 5 days
Treatment:Dietary
Treatment Compound:TCDF
Treatment Route:Oral
Treatment Dose:24 µg/kg body weight for AHR and 5 µg/kg body weight for wild type

Sample Preparation:

Sampleprep ID:SP000247
Sampleprep Summary:-
Sampleprep Protocol Filename:NMR_metabolomics_protocol.docx
Processing Method:Homogenization
Processing Storage Conditions:-20
Extraction Method:Methanol/Water
Extract Concentration Dilution:2 Methanol : 1 Water
Sample Resuspension:D2O
Sample Spiking:TSP-d4
Organ:Liver, small and large intestine

Analysis:

MB Sample ID:SA010809
Analysis ID:AN000336
Analysis Type:NMR
Chromatography ID:CH000252
Num Factors:4

NMR:

NMR ID:NM000055
Analysis ID:AN000336
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:NOESY PR1D
Spectrometer Frequency:600 MHz
NMR Solvent:D2O
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