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MB Sample ID: SA084791
Local Sample ID: | 1-hr cold 4 |
Subject ID: | SU001273 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Subject:
Subject ID: | SU001273 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
1-hr cold 4 | SA084791 | FL012820 | 5C | Temperature |
1-hr cold 4 | SA084791 | FL012820 | 1 hour | Time of exposure |
Collection:
Collection ID: | CO001267 |
Collection Summary: | Mice were anaesthethized with isoflurane, the blood collected through cardiac puncture, and left under room temperature for 30 minutes. After that, the blood was centrifuged for 3 minutes at 14,000 rpm. Then, serum was transferred for a clean 1.5ml microbe and frost. |
Sample Type: | Blood (serum) |
Storage Conditions: | -20℃ |
Treatment:
Treatment ID: | TR001288 |
Treatment Summary: | We divided the C57BL6/J mice in 6 groups: females housed in 30C degree for 1 week and females housed in 5C degree for 1 week; Males housed in 30C degree for 1 week and females housed in 5C degree for 1 week; males housed in 22C for 1 hour and males housed in 5C for 1 hour. |
Sample Preparation:
Sampleprep ID: | SP001281 |
Sampleprep Summary: | Aliquots of 100 µL serum or were taken. A mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H2O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H2O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LC–MS/MS mediator lipidomics platform. |
Combined analysis:
Analysis ID | AN002008 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Ekspert MicroLC 200 system |
Column | Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um) |
MS Type | ESI |
MS instrument type | Triple TOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001452 |
Instrument Name: | Ekspert MicroLC 200 system |
Column Name: | Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001861 |
Analysis ID: | AN002008 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Full-scan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX). |
Ion Mode: | NEGATIVE |