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MB Sample ID: SA151662
Local Sample ID: | shoot-8 |
Subject ID: | SU001725 |
Subject Type: | Plant |
Subject Species: | Hordeum vulgare L. |
Taxonomy ID: | 4513 |
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Subject:
Subject ID: | SU001725 |
Subject Type: | Plant |
Subject Species: | Hordeum vulgare L. |
Taxonomy ID: | 4513 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
shoot-8 | SA151662 | FL017796 | Shoot_Salt-4 | Treatment |
Collection:
Collection ID: | CO001718 |
Collection Summary: | Barley seedlings were allowed to grow up to two-leaf-stage in the normal hydroponic solution, and then exposed to various treatments after removing endosperm for eliminating its nutrient supply. The treatments included 20% PEG for drought stress, 150 mM NaCl for salt stress, 50 μM Al3+ at pH 4.5 for aluminum stress (Al stress, at the same time the nutrient solution with pH 4.5 and without Al addition used as control), 5 μM CdCl2 for cadmium stress (Cd stress), 0 mM N for N deficiency (replace KNO3, Ca(NO3).4H2O and (NH4)6Mo7O24 with 1mM CaCl2, 1mM KH2PO4 and 0.07 mM H2MoO4), 0 mM P for P deficiency (replace NH4H2PO4 with 1mM NH4Cl), 0 mM K for K deficiency (replace KNO3 with 1mM NaNO3). The hydroponic solution was renewed every four days. After twenty days of the treatments, the plants were harvested, and fresh and dry weight were recorded quickly. The dry tissues were used for mineral element concentration analysis with three biological replicates for each treatment. Meanwhile, fresh tissues were frozen in liquid nitrogen and stored at -80 ℃ for metabolite analysis with four biological replicates for each stress treatment. |
Sample Type: | Plant |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001738 |
Treatment Summary: | The treatments included 20% PEG for drought stress, 150 mM NaCl for salt stress, 50 μM Al3+ at pH 4.5 for aluminum stress (Al stress, at the same time the nutrient solution with pH 4.5 and without Al addition used as control), 5 μM CdCl2 for cadmium stress (Cd stress), 0 mM N for N deficiency (replace KNO3, Ca(NO3).4H2O and (NH4)6Mo7O24 with 1mM CaCl2, 1mM KH2PO4 and 0.07 mM H2MoO4), 0 mM P for P deficiency (replace NH4H2PO4 with 1mM NH4Cl), 0 mM K for K deficiency (replace KNO3 with 1mM NaNO3). |
Sample Preparation:
Sampleprep ID: | SP001731 |
Sampleprep Summary: | The frozen tissue was homogenized in liquid nitrogen and approximately 100 mg of powder sample was added into 2 ml vertical centrifuge tube. Then 1.4 ml of 100% methanol (pre-cooled at -20 °C) was added and vortexed thoroughly. Then the tubes were shaken for 10 min at 70 °C, followed by centrifugation at 11,000 g for 10 min. The supernatant was transferred into a new 10 ml sterile centrifuge tube, and 750 μl chloroform (−20 °C) and 1.5 ml deionized water (4 °C) were added. The mixture was vortexed and centrifuged at 2200 g for 15 min. The upper methanol/water phase was collected for metabolite analysis. A total of 150 μl collected solution was dried in a vacuum freeze dryer. The dried residue was derivatized in 40 μl of 15 mg ml-1 methoxylamine hydrochloride in pyridine for 120 min at 37 °C, followed by reaction with 70 μl of N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) reagent for another 30 min at 37 °C. After the reaction, the reacted solution was transferred to glass vial insert in 2 ml glass bottle (Agilent, USA) for GC-MS determination. Meanwhile, one blank derivatization reaction (with reagent) was also prepared as a control. |
Combined analysis:
Analysis ID | AN002694 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Agilent 7890A |
Ion Mode | UNSPECIFIED |
Units | pmoles/l |
Chromatography:
Chromatography ID: | CH001987 |
Instrument Name: | Agilent 7890A |
Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS002492 |
Analysis ID: | AN002694 |
Instrument Name: | Agilent 7890A |
Instrument Type: | GC-TOF |
MS Type: | EI |
MS Comments: | The retention time (RT) and mass spectra of each compound were analyzed using AMDIS (Automated Mass Spectral Deconvolution and Identification System) software (http://chemdata.nist.gov/). |
Ion Mode: | UNSPECIFIED |