Return to study ST001863 main page
MB Sample ID: SA174430
Local Sample ID: | Caco2_bxa2 |
Subject ID: | SU001940 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | HT-29 and Caco-2 cells |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001940 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | HT-29 and Caco-2 cells |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Caco2_bxa2 | SA174430 | FL021426 | Bxa | Treatment |
Collection:
Collection ID: | CO001933 |
Collection Summary: | Cell supernatants were collected by centrifugation of the epithelial cells and frozen at -80 degrees until analysis. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR001952 |
Treatment Summary: | Cells were treated with 200nM Bxa protein and compared to a control of the same buffer but without protein. |
Sample Preparation:
Sampleprep ID: | SP001946 |
Sampleprep Summary: | Samples were prepped by adding isopropanol to the supernatant to extract metabolites and then injected on the machine |
Combined analysis:
Analysis ID | AN003020 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | ID-X |
Column | C8 |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Area under curve |
Chromatography:
Chromatography ID: | CH002238 |
Instrument Name: | ID-X |
Column Name: | C8 |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002809 |
Analysis ID: | AN003020 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Data were analyzed using Progenesis QI peak picking software ThermoScientific Trace Finder 5.0. |
Ion Mode: | POSITIVE |