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MB Sample ID: SA330233
| Local Sample ID: | Set1-BMS-C |
| Subject ID: | SU003154 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003154 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Set1-BMS-C | SA330233 | FL039073 | BMS309403 | Treatment |
Collection:
| Collection ID: | CO003147 |
| Collection Summary: | Cells were collected, centrifuged, and washed X3 in cold PBS before lipid isolation. Tumors were removed from mice and immediately flash frozen by submersion in Liquid Nitrogen and transfer on dry ice for lipid isolation. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR003163 |
| Treatment Summary: | MM.1S Myeloma tumor cells were treated with 50 μM BMS309403 once over 24 hours in vitro or at 5mg/kg X3 per week over 32 days in vivo. In Vivo tumors were made by subcutaneously injecting 1M Luc+/GFP+ MM.1S cells mixed with Matrigel in a 1:1 ratio into the backs of 8-week old, female SCID-Beige. Vehicle (PBS with a matched concentration of 5% DMSO) was used as control for each condition. |
Sample Preparation:
| Sampleprep ID: | SP003160 |
| Sampleprep Summary: | Lipids were extracted from cell and tumors previously treated with Vehicle and BMS309403 using a modified Bligh and Dyer protocol [Bligh and Dyer, 1959] of Avanti Polar Lipids, INC. Water:Methanol:Dichloromethane (34:34:31, v/v/v). After the extraction and dried out the samples were resuspended in Methanol:Dichloromethane (50:50, v/v) with 10 mM of Ammonium acetate to be injected by Direct Infusion. |
Combined analysis:
| Analysis ID | AN004987 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | AB Sciex 4000 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | Corrected Peak intensities |
Chromatography:
| Chromatography ID: | CH003767 |
| Chromatography Summary: | Direct Infusion with PAL3 System |
| Instrument Name: | none |
| Column Name: | none |
| Column Temperature: | None |
| Flow Gradient: | None |
| Flow Rate: | None |
| Solvent A: | None |
| Solvent B: | None |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS004727 |
| Analysis ID: | AN004987 |
| Instrument Name: | AB Sciex 4000 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | The analysis was made in POSITIVE and NEGATIVE polarities. Extract MS/MS data: Fragments_POS= Mass tolerance (Da) 0.3, Fragments_NEG= Mass tolerance (Da) 0.5 spectrum peak. Source parameters: GS1=15, GS2=20, CUR= 10, ISV=5300, T=150. Lipids were analyzed in multiple precursor ion scattering (MPIS). Identification of Lipids was using LipidView 1.3 beta (Sciex) and exported to MarkerView 1.4 in a view mode: Corrected intensities (normalization with the high peak intensity) |
| Ion Mode: | UNSPECIFIED |
