Summary of Study ST003040

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001892. The data can be accessed directly via it's Project DOI: 10.21228/M8572M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003040
Study TitleMM.1S Myeloma tumor cells and tumors made by subcutaneously injecting 1M Luc+/GFP+ MM.1S cells treated with 50 μM BMS309403 and Vehicle (PBS with a matched concentration of 5% DMSO)
Study SummaryMM.1S Myeloma tumor cells were treated with 50 μM BMS309403 once over 24 hours in vitro or at 5mg/kg X3 per week over 32 days in vivo. In Vivo tumors were made by subcutaneously injecting 1M Luc+/GFP+ MM.1S cells mixed with Matrigel in a 1:1 ratio into the backs of 8-week old, female SCID-Beige. Vehicle (PBS with a matched concentration of 5% DMSO) was used as control for each condition.
Institute
Mainehealth
Last NameReagan
First NameMichaela
Address81 Research Drive, Scarborough, ME, Portland, ME, 04074, USA
EmailMichaela.Reagan@mainehealth.org
Phone(207)396-8196
Submit Date2023-12-20
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2024-03-25
Release Version1
Michaela Reagan Michaela Reagan
https://dx.doi.org/10.21228/M8572M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001892
Project DOI:doi: 10.21228/M8572M
Project Title:Lipid analysis of BMS309403-treated myeloma cells in vitro and in vivo
Project Summary:MM.1S Myeloma tumor cells were treated with 50 μM BMS309403 once over 24 hours in vitro or at 5mg/kg X3 per week over 32 days in vivo. In Vivo tumors were made by subcutaneously injecting 1M Luc+/GFP+ MM.1S cells mixed with Matrigel in a 1:1 ratio into the backs of 8-week old, female SCID-Beige. Vehicle (PBS with a matched concentration of 5% DMSO) was used as control for each condition.
Institute:Mainehealth
Last Name:Reagan
First Name:Michaela
Address:81 Research Drive, Scarborough, ME, Portland, ME, 04074, USA
Email:Michaela.Reagan@mainehealth.org
Phone:(207)396-8196

Subject:

Subject ID:SU003154
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA330226Set2-BMS-CBMS309403
SA330227Set2-BMS-ABMS309403
SA330228Set2-BMS-BBMS309403
SA330229Set3-BMS-CBMS309403
SA330230Set3-BMS-ABMS309403
SA330231C2L-ABMS309403
SA330232Set1-BMS-BBMS309403
SA330233Set1-BMS-CBMS309403
SA330234Set3-BMS-BBMS309403
SA330235C7R-BBMS309403
SA330236C2R-BBMS309403
SA330237C2R-ABMS309403
SA330238C2L-BBMS309403
SA330239C4L-ABMS309403
SA330240C4L-BBMS309403
SA330241C7R-ABMS309403
SA330242C4R-BBMS309403
SA330243C4R-ABMS309403
SA330244Set1-BMS-ABMS309403
SA330245Set3-Veh-BControl
SA330246Set3-Veh-CControl
SA330247Set3-Veh-AControl
SA330248Set2-Veh-CControl
SA330249Set2-Veh-BControl
SA330250Set2-Veh-AControl
SA330251C8R-AControl
SA330252C6B-AControl
SA330253C6B-BControl
SA330254C1R-BControl
SA330255C1B-BControl
SA330256C1B-AControl
SA330257C6N-AControl
SA330258C6N-BControl
SA330259Set1-Veh-AControl
SA330260Set1-Veh-BControl
SA330261C8R-BControl
SA330262C8L-BControl
SA330263C8L-AControl
SA330264Set1-Veh-CControl
Showing results 1 to 39 of 39

Collection:

Collection ID:CO003147
Collection Summary:Cells were collected, centrifuged, and washed X3 in cold PBS before lipid isolation. Tumors were removed from mice and immediately flash frozen by submersion in Liquid Nitrogen and transfer on dry ice for lipid isolation.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR003163
Treatment Summary:MM.1S Myeloma tumor cells were treated with 50 μM BMS309403 once over 24 hours in vitro or at 5mg/kg X3 per week over 32 days in vivo. In Vivo tumors were made by subcutaneously injecting 1M Luc+/GFP+ MM.1S cells mixed with Matrigel in a 1:1 ratio into the backs of 8-week old, female SCID-Beige. Vehicle (PBS with a matched concentration of 5% DMSO) was used as control for each condition.

Sample Preparation:

Sampleprep ID:SP003160
Sampleprep Summary:Lipids were extracted from cell and tumors previously treated with Vehicle and BMS309403 using a modified Bligh and Dyer protocol [Bligh and Dyer, 1959] of Avanti Polar Lipids, INC. Water:Methanol:Dichloromethane (34:34:31, v/v/v). After the extraction and dried out the samples were resuspended in Methanol:Dichloromethane (50:50, v/v) with 10 mM of Ammonium acetate to be injected by Direct Infusion.

Combined analysis:

Analysis ID AN004987
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type ESI
MS instrument type QTRAP
MS instrument name AB Sciex 4000 QTrap
Ion Mode UNSPECIFIED
Units Corrected Peak intensities

Chromatography:

Chromatography ID:CH003767
Chromatography Summary:Direct Infusion with PAL3 System
Instrument Name:none
Column Name:none
Column Temperature:None
Flow Gradient:None
Flow Rate:None
Solvent A:None
Solvent B:None
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS004727
Analysis ID:AN004987
Instrument Name:AB Sciex 4000 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:The analysis was made in POSITIVE and NEGATIVE polarities. Extract MS/MS data: Fragments_POS= Mass tolerance (Da) 0.3, Fragments_NEG= Mass tolerance (Da) 0.5 spectrum peak. Source parameters: GS1=15, GS2=20, CUR= 10, ISV=5300, T=150. Lipids were analyzed in multiple precursor ion scattering (MPIS). Identification of Lipids was using LipidView 1.3 beta (Sciex) and exported to MarkerView 1.4 in a view mode: Corrected intensities (normalization with the high peak intensity)
Ion Mode:UNSPECIFIED
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