Summary of Study ST002086

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001325. The data can be accessed directly via it's Project DOI: 10.21228/M8FM6D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002086
Study TitleSerum NMR profiling reveals differential alterations in the lipoproteome induced by Pfizer-BioNTech vaccine in COVID-19–recovered subjects and naïve subjects
Study TypeNMR-based metabolomics
Study Summary1H NMR spectra of sera have been used to define the changes induced by vaccination with Pfizer-BioNTech vaccine (2 shots, 21 days apart) in 10 COVID-19-recovered subjects and 10 COVID-19-naïve subjects at different time points, starting from before vaccination, then weekly until 7 days after second injection, and finally 1 month after the second dose. The data show that vaccination does not induce any significant variation in the metabolome, whereas it causes changes at the level of lipoproteins. The effects are different in the COVID-19-recovered subjects with respect to the naïve subjects, suggesting that a previous infection reduces the vaccine modulation of the lipoproteome composition.
Institute
University of Florence
Last NameGhini
First NameVeronica
Addressvia Luigi Sacconi
Emailghini@cerm.unifi.it
Phone03922800462
Submit Date2022-02-14
Num Groups2
Total Subjects20
Num Males7
Num Females13
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2022-02-28
Release Version1
Veronica Ghini Veronica Ghini
https://dx.doi.org/10.21228/M8FM6D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001325
Project DOI:doi: 10.21228/M8FM6D
Project Title:Serum NMR profiling reveals differential alterations in the lipoproteome induced by Pfizer-BioNTech vaccine in COVID-19–recovered subjects and naïve subjects
Project Type:NMR-based metabolomic analysis
Project Summary:1H NMR spectra of sera have been used to define the changes induced by vaccination with Pfizer-BioNTech vaccine (2 shots, 21 days apart) in 10 COVID-19-recovered subjects and 10 COVID-19-naïve subjects at different time points, starting from before vaccination, then weekly until 7 days after second injection, and finally 1 month after the second dose. The data show that vaccination does not induce any significant variation in the metabolome, whereas it causes changes at the level of lipoproteins. The effects are different in the COVID-19-recovered subjects with respect to the naïve subjects, suggesting that a previous infection reduces the vaccine modulation of the lipoproteome composition.
Institute:University of Florence
Department:Department of chemistry
Laboratory:Metabolomics
Last Name:Ghini
First Name:Veronica
Address:via Luigi Sacconi 6
Email:ghini@cerm.unifi.it
Phone:+390554574266

Subject:

Subject ID:SU002170
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:24-65
Gender:Male and female
Human Ethnicity:Caucasian

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Previus_COVID
SA19832065-Serum_MF_T0No | TIME:T0
SA1983211-Serum_AF_T0No | TIME:T0
SA19832253-Serum_GT_T0No | TIME:T0
SA19832371-Serum_MC_T0No | TIME:T0
SA19832488-Serum_PS_T0No | TIME:T0
SA198325111-Serum_VS_T0No | TIME:T0
SA19832694-Serum_RA_T0No | TIME:T0
SA19832730-Serum_CMG_T0No | TIME:T0
SA19832859-Serum_KT_T0No | TIME:T0
SA1983297-Serum_CI_T0No | TIME:T0
SA19833067-Serum_MF_T14No | TIME:T14
SA19833132-Serum_CMG_T14No | TIME:T14
SA19833261-Serum_KT_T14No | TIME:T14
SA1983339-Serum_CI_T14No | TIME:T14
SA19833455-Serum_GT_T14No | TIME:T14
SA19833590-Serum_PS_T14No | TIME:T14
SA19833673-Serum_MC_T14No | TIME:T14
SA1983373-Serum_AF_T14No | TIME:T14
SA19833896-Serum_RA_T14No | TIME:T14
SA198339113-Serum_VS_T14No | TIME:T14
SA19834058-Serum_GT_T1MNo | TIME:T1M
SA198341116-Serum_VS_T1MNo | TIME:T1M
SA19834235-Serum_CMG_T1MNo | TIME:T1M
SA19834399-Serum_RA_T1MNo | TIME:T1M
SA19834493-Serum_PS_T1MNo | TIME:T1M
SA1983456-Serum_AF_T1MNo | TIME:T1M
SA19834670-Serum_MF_T1MNo | TIME:T1M
SA19834776-Serum_MC_T1MNo | TIME:T1M
SA19834864-Serum_KT_T1MNo | TIME:T1M
SA19834912-Serum_CI_T1MNo | TIME:T1M
SA19835068-Serum_MF_T21No | TIME:T21
SA19835162-Serum_KT_T21No | TIME:T21
SA19835291-Serum_PS_T21No | TIME:T21
SA19835397-Serum_RA_T21No | TIME:T21
SA198354114-Serum_VS_T21No | TIME:T21
SA19835556-Serum_GT_T21No | TIME:T21
SA1983564-Serum_AF_T21No | TIME:T21
SA19835774-Serum_MC_T21No | TIME:T21
SA19835833-Serum_CMG_T21No | TIME:T21
SA19835910-Serum_CI_T21_200ulNo | TIME:T21
SA19836075-Serum_MC_T28No | TIME:T28
SA19836163-Serum_KT_T28_280ulNo | TIME:T28
SA19836234-Serum_CMG_T28No | TIME:T28
SA19836369-Serum_MF_T28No | TIME:T28
SA19836411-Serum_CI_T28No | TIME:T28
SA1983655-Serum_AF_T28No | TIME:T28
SA198366115-Serum_VS_T28No | TIME:T28
SA19836792-Serum_PS_T28No | TIME:T28
SA19836857-Serum_GT_T28No | TIME:T28
SA19836998-Serum_RA_T28No | TIME:T28
SA19837060-Serum_KT_T07No | TIME:T7
SA19837172-Serum_MC_T07No | TIME:T7
SA19837295-Serum_RA_T07_250uLNo | TIME:T7
SA19837389-Serum_PS_T7No | TIME:T7
SA198374112-Serum_VS_T07No | TIME:T7
SA1983758-Serum_CI_T07No | TIME:T7
SA19837666-Serum_MF_T7No | TIME:T7
SA19837731-Serum_CMG_T7No | TIME:T7
SA19837854-Serum_GT_T7No | TIME:T7
SA1983792-Serum_AF_T7No | TIME:T7
SA19838083-Serum_PF_T0Yes | TIME:T0
SA198381100-Serum_SA_T0Yes | TIME:T0
SA19838219-Serum_CCa_T07Yes | TIME:T0
SA19838313-Serum_CC_T07_250uLYes | TIME:T0
SA19838448-Serum_DD_T0Yes | TIME:T0
SA19838577-Serum_NF_T0Yes | TIME:T0
SA198386106-Serum_SB_T0Yes | TIME:T0
SA19838742-Serum_CA_T0Yes | TIME:T0
SA19838825-Serum_CS_T0Yes | TIME:T0
SA19838936-Serum_CG_T0Yes | TIME:T0
SA19839044-Serum_CA_T14Yes | TIME:T14
SA19839179-Serum_NF_T14Yes | TIME:T14
SA19839215-Serum_CC_T21Yes | TIME:T14
SA198393107-Serum_SB_T14Yes | TIME:T14
SA19839427-Serum_CS_T14Yes | TIME:T14
SA19839585-Serum_PF_T14Yes | TIME:T14
SA198396102-Serum_SA_T14Yes | TIME:T14
SA19839721-Serum_CCa_T21Yes | TIME:T14
SA19839838-Serum_CG_T14Yes | TIME:T14
SA19839987-Serum_PF_T1MYes | TIME:T1M
SA19840041-Serum_CG_T1MYes | TIME:T1M
SA198401105-Serum_SA_T1MYes | TIME:T1M
SA19840218-Serum_CC_T1MYes | TIME:T1M
SA19840347-Serum_CA_T1MYes | TIME:T1M
SA19840452-Serum_DD_T1MYes | TIME:T1M
SA19840524-Serum_CCa_T1MYes | TIME:T1M
SA19840682-Serum_NF_T1MYes | TIME:T1M
SA198407110-Serum_SB_T1MYes | TIME:T1M
SA19840845-Serum_CA_T21Yes | TIME:T21
SA19840950-Serum_DD_T21Yes | TIME:T21
SA198410103-Serum_SA_T21Yes | TIME:T21
SA19841128-Serum_CS_T21Yes | TIME:T21
SA198412108-Serum_SB_T21Yes | TIME:T21
SA19841339-Serum_CG_T21Yes | TIME:T21
SA19841486-Serum_PF_T21Yes | TIME:T21
SA19841522-Serum_CCa_T28Yes | TIME:T21
SA19841680-Serum_NF_T21Yes | TIME:T21
SA19841716-Serum_CC_T28Yes | TIME:T21
SA19841817-Serum_CC_T0_250uLYes | TIME:T28
SA19841981-Serum_NF_T28Yes | TIME:T28
Showing page 1 of 2     Results:    1  2  Next     Showing results 1 to 100 of 116

Collection:

Collection ID:CO002163
Collection Summary:For all subjects blood serum samples were collected at six different time points: before the first dose (T0); 7 and 14 days after the first dose (T7 and T14, respectively); 21 days after the first dose, just before the second dose (T21); 28 days after the first dose and 7 days after the second dose (T28); 1 month after the second dose (T1M). Blood samples were collected (4 hours after breakfast) in a BD vacutainer clot-activator tube for serum collection and processed within one hour from sample collection. After processing, all the serum samples were immediately stored at -30°C until NMR analysis.
Sample Type:Blood (serum)
Storage Conditions:Described in summary
Collection Vials:BD vacutainer clot-activator tube for serum

Treatment:

Treatment ID:TR002182
Treatment Summary:We monitor the effect of mRNA Pfizer-BioNTech vaccine in a cohort of 20 healthcare workers. All of them received two doses, 21 days apart.
Treatment:Pfizer-BioNTech mRNA vaccine

Sample Preparation:

Sampleprep ID:SP002176
Sampleprep Summary:NMR samples were prepared according to standard procedures. Frozen serum samples were thawed at room temperature. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis.
Processing Storage Conditions:-80℃

Analysis:

Analysis ID:AN003404
Analysis Type:NMR
Num Factors:12
Num Metabolites:114
Units:mM

NMR:

NMR ID:NM000230
Analysis ID:AN003404
Instrument Name:Bruker
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600MHz
NMR Probe:5 mm PATXI 1H−13C−15N and 2H-decoupling probe
NMR Tube Size:5.00 mm
Pulse Sequence:NOESY
Water Suppression:1Dpresat
Temperature:36,85
Number Of Scans:32
Dummy Scans:4
Acquisition Time:2.7 s
Relaxation Delay:4 s
Spectral Width:18,028 Hz
Num Data Points Acquired:98,304
Line Broadening:0.3
Binned Increment:0.02 ppm
Binned Data Excluded Range:4.5-5.5 ppm
NMR Results File:NOESY.txt UNITS:ppm
  logo