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MB Sample ID: SA006635
Local Sample ID: | H31 |
Subject ID: | SU000140 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 20 weeks |
Weight Or Weight Range: | 26.8 +/- 2.2 g |
Gender: | Male |
Animal Animal Supplier: | Jackson Labs (Bar Harbor, ME) |
Animal Housing: | 8 / cage |
Animal Light Cycle: | 12 h / 12 h light/dark |
Animal Feed: | Standard chow, ad lib. |
Animal Water: | Ad lib. Access |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000203 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1200 |
Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | TOF |
MS instrument name | Agilent 6220 TOF |
Ion Mode | NEGATIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000135 |
Chromatography Summary: | Polar metabolites were analyzed by HILIC-MS using an aminopropyl stationary phase |
Instrument Name: | Agilent 1200 |
Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
Column Pressure: | 150 bar |
Column Temperature: | 25 C |
Flow Gradient: | 80-0% B over 20 minutes, hold 4 minutes, return to 80%B |
Flow Rate: | 0.25 mL/min |
Sample Injection: | 25 uL |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Analytical Time: | 32 min |
Chromatography Type: | HILIC |