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MB Sample ID: SA333162
| Local Sample ID: | Norm. Area 088_0009_01B_R_TM9xWTxTAx24x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F281) |
| Subject ID: | SU003206 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005056 | AN005057 | AN005058 | AN005059 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003819 |
| Chromatography Summary: | Reversed phase chromatography |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) |
| Column Temperature: | 45 |
| Flow Gradient: | 0%A for 1 min, 50% B for 4 min, 98%B for 3 min, 0% A for 5min |
| Flow Rate: | 0.3ml/min |
| Injection Temperature: | 4 |
| Solvent A: | Water, 0.1% formic acid |
| Solvent B: | Acetonitrile, 0.1% formic acid |
| Transferline Temperature: | 275 |
| Target Sample Temperature: | 4 |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003820 |
| Chromatography Summary: | HILIC chromatography |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | 99%B for 1min, 85%B for 2min, 75%B for 3 min, 30%B for 3 min, 99%B for 5 min |
| Flow Rate: | 0.35ml/min |
| Injection Temperature: | 4 |
| Solvent A: | 10mM ammonium acetate, 95% Water, 5% Acetonitrile, 0.1% acetic acid |
| Solvent B: | 10mM ammonium acetate, 95% Acetonitrile, 95% Water, 0.1% acetic acid |
| Transferline Temperature: | 275 |
| Target Sample Temperature: | 4 |
| Chromatography Type: | HILIC |
