Summary of Study ST003091

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001920. The data can be accessed directly via it's Project DOI: 10.21228/M8JB09 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003091
Study Title	T cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia
Study Type	Untargeted metabolomics
Study Summary	We report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models.
Institute	Translational Genomics Research Institute
Department	Integrated Mass Spectrometry Shared Resources
Last Name	Pirrotte
First Name	Patrick
Address	445 N 5th St Phoenix, AZ 85004
Email	ppirrotte@tgen.org
Phone	6023438400
Submit Date	2024-01-26
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-03-01
Release Version	1

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Project:

Project ID:	PR001920
Project DOI:	doi: 10.21228/M8JB09
Project Title:	T cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia
Project Type:	Untargeted metabolomics
Project Summary:	We report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models.
Institute:	Translational Genomics Research Institute
Department:	Integrated Mass Spectrometry Shared Resources
Last Name:	Pirrotte
First Name:	Patrick
Address:	445 N 5th St Phoenix, AZ 85004
Email:	ppirrotte@tgen.org
Phone:	6023438400
Contributors:	Bin Zhang, Khyatiben V. Pathak, Brooke Lovell, Nate Hansen, Patrick Pirrote, Le Xuan Truong Nguyen, Guido Marcucci,

Subject:

Subject ID:	SU003206
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Genotype
SA332985	Norm. Area 088_0009_01B_R_TM48xWTxCMLxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F320)	T cells	KO
SA332986	Norm. Area 088_0009_01B_R_TM49xWTxCMLxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F321)	T cells	KO
SA332987	Norm. Area 088_0009_01B_R_TM50xWTxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F322)	T cells	KO
SA332988	Norm. Area 088_0009_01B_R_TM47xWTxCMLxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F319)	T cells	KO
SA332989	Norm. Area 088_0009_01B_R_TM45xKOxCMLxTAx24x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F317)	T cells	KO
SA332990	Norm. Area 088_0009_01B_R_TM43xKOxCMLxTAx24x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F315)	T cells	KO
SA332991	Norm. Area 088_0009_01B_R_TM44xKOxCMLxTAx24x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F316)	T cells	KO
SA332992	Norm. Area 088_0009_01B_R_TM51xWTxCMLxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F323)	T cells	KO
SA332993	Norm. Area 088_0009_01B_R_TM46xKOxCMLxTAx24x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F318)	T cells	KO
SA332994	Norm. Area 088_0009_01B_R_TM53xKOxCMLxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F325)	T cells	KO
SA332995	Norm. Area 088_0009_01D_R_TM21xWTxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F193)	T cells	KO
SA332996	Norm. Area 088_0009_01D_R_TM20xWTxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F192)	T cells	KO
SA332997	Norm. Area 088_0009_01D_R_TM19xWTxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F191)	T cells	KO
SA332998	Norm. Area 088_0009_01D_R_TM22xWTxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F194)	T cells	KO
SA332999	Norm. Area 088_0009_01B_R_TM56xKOxCMLxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F328)	T cells	KO
SA333000	Norm. Area 088_0009_01B_R_TM42xKOxCMLxTFx5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F314)	T cells	KO
SA333001	Norm. Area 088_0009_01B_R_TM54xKOxCMLxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F326)	T cells	KO
SA333002	Norm. Area 088_0009_01B_R_TM55xKOxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F327)	T cells	KO
SA333003	Norm. Area 088_0009_01B_R_TM52xKOxCMLxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F324)	T cells	KO
SA333004	Norm. Area 088_0009_01B_R_TM41xKOxCMLxTFx4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F313)	T cells	KO
SA333005	Norm. Area 088_0009_01C_R_TM51xWTxCMLxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F135)	T cells	KO
SA333006	Norm. Area 088_0009_01C_R_TM50xWTxCMLxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F134)	T cells	KO
SA333007	Norm. Area 088_0009_01C_R_TM49xWTxCMLxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F133)	T cells	KO
SA333008	Norm. Area 088_0009_01B_R_TM28xKOxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F300)	T cells	KO
SA333009	Norm. Area 088_0009_01B_R_TM27xKOxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F299)	T cells	KO
SA333010	Norm. Area 088_0009_01B_R_TM24xKOxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F296)	T cells	KO
SA333011	Norm. Area 088_0009_01B_R_TM25xKOxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F297)	T cells	KO
SA333012	Norm. Area 088_0009_01B_R_TM26xKOxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F298)	T cells	KO
SA333013	Norm. Area 088_0009_01D_R_TM28xKOxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F200)	T cells	KO
SA333014	Norm. Area 088_0009_01D_R_TM27xKOxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F199)	T cells	KO
SA333015	Norm. Area 088_0009_01B_R_TM38xKOxCMLxTFx1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F310)	T cells	KO
SA333016	Norm. Area 088_0009_01B_R_TM39xKOxCMLxTFx2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F311)	T cells	KO
SA333017	Norm. Area 088_0009_01B_R_TM40xKOxCMLxTFx3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F312)	T cells	KO
SA333018	Norm. Area 088_0009_01D_R_TM23xWTxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F195)	T cells	KO
SA333019	Norm. Area 088_0009_01D_R_TM24xKOxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F196)	T cells	KO
SA333020	Norm. Area 088_0009_01D_R_TM26xKOxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F198)	T cells	KO
SA333021	Norm. Area 088_0009_01D_R_TM25xKOxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F197)	T cells	KO
SA333022	Norm. Area 088_0009_01D_R_TM18xKOxTAx24x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F190)	T cells	KO
SA333023	Norm. Area 088_0009_01D_R_TM17xKOxTAx24x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F189)	T cells	KO
SA333024	Norm. Area 088_0009_01D_R_TM11xKOxTFx2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F183)	T cells	KO
SA333025	Norm. Area 088_0009_01D_R_TM10xKOxTFx1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F182)	T cells	KO
SA333026	Norm. Area 088_0009_01C_R_TM48xWTxCMLxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F132)	T cells	KO
SA333027	Norm. Area 088_0009_01D_R_TM12xKOxTFx3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F184)	T cells	KO
SA333028	Norm. Area 088_0009_01D_R_TM13xKOxTFx4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F185)	T cells	KO
SA333029	Norm. Area 088_0009_01C_R_TM26xKOxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F110)	T cells	KO
SA333030	Norm. Area 088_0009_01C_R_TM27xKOxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F111)	T cells	KO
SA333031	Norm. Area 088_0009_01C_R_TM28xKOxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F112)	T cells	KO
SA333032	Norm. Area 088_0009_01C_R_TM47xWTxCMLxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F131)	T cells	KO
SA333033	Norm. Area 088_0009_01C_R_TM46xKOxCMLxTAx24x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F130)	T cells	KO
SA333034	Norm. Area 088_0009_01C_R_TM40xKOxCMLxTFx3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F124)	T cells	KO
SA333035	Norm. Area 088_0009_01C_R_TM41xKOxCMLxTFx4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F125)	T cells	KO
SA333036	Norm. Area 088_0009_01C_R_TM43xKOxCMLxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F126)	T cells	KO
SA333037	Norm. Area 088_0009_01C_R_TM39xKOxCMLxTFx2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F123)	T cells	KO
SA333038	Norm. Area 088_0009_01C_R_TM38xKOxCMLxTFx1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F122)	T cells	KO
SA333039	Norm. Area 088_0009_01C_R_TM45xKOxCMLxTAx24x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F129)	T cells	KO
SA333040	Norm. Area 088_0009_01C_R_TM44xKOxCMLxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F128)	T cells	KO
SA333041	Norm. Area 088_0009_01C_R_TM25xKOxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F109)	T cells	KO
SA333042	Norm. Area 088_0009_01C_R_TM24xKOxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F108)	T cells	KO
SA333043	Norm. Area 088_0009_01C_R_TM12xKOxTFx3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F96)	T cells	KO
SA333044	Norm. Area 088_0009_01C_R_TM13xKOxTFx4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F97)	T cells	KO
SA333045	Norm. Area 088_0009_01C_R_TM14xKOxTFx5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F98)	T cells	KO
SA333046	Norm. Area 088_0009_01C_R_TM11xKOxTFx2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F95)	T cells	KO
SA333047	Norm. Area 088_0009_01C_R_TM10xKOxTFx1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F94)	T cells	KO
SA333048	Norm. Area 088_0009_01D_R_TM16xKOxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F188)	T cells	KO
SA333049	Norm. Area 088_0009_01D_R_TM15xKOxTAx24x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F187)	T cells	KO
SA333050	Norm. Area 088_0009_01D_R_TM14xKOxTFx5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F186)	T cells	KO
SA333051	Norm. Area 088_0009_01C_R_TM15xKOxTAx24x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F99)	T cells	KO
SA333052	Norm. Area 088_0009_01C_R_TM16xKOxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F100)	T cells	KO
SA333053	Norm. Area 088_0009_01C_R_TM21xWTxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F105)	T cells	KO
SA333054	Norm. Area 088_0009_01C_R_TM22xWTxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F106)	T cells	KO
SA333055	Norm. Area 088_0009_01C_R_TM23xWTxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F107)	T cells	KO
SA333056	Norm. Area 088_0009_01C_R_TM20xWTxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F104)	T cells	KO
SA333057	Norm. Area 088_0009_01C_R_TM19xWTxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F103)	T cells	KO
SA333058	Norm. Area 088_0009_01C_R_TM17xKOxTAx24x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F101)	T cells	KO
SA333059	Norm. Area 088_0009_01C_R_TM18xKOxTAx24x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F102)	T cells	KO
SA333060	Norm. Area 088_0009_01B_R_TM23xWTxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F295)	T cells	KO
SA333061	Norm. Area 088_0009_01B_R_TM21xWTxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F293)	T cells	KO
SA333062	Norm. Area 088_0009_01A_R_TM27xKOxTAx72x4xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F39)	T cells	KO
SA333063	Norm. Area 088_0009_01A_R_TM28xKOxTAx72x5xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F40)	T cells	KO
SA333064	Norm. Area 088_0009_01D_R_TM49xWTxCMLxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F222)	T cells	KO
SA333065	Norm. Area 088_0009_01A_R_TM26xKOxTAx72x3xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F38)	T cells	KO
SA333066	Norm. Area 088_0009_01A_R_TM25xKOxTAx72x2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F37)	T cells	KO
SA333067	Norm. Area 088_0009_01A_R_TM22xWTxTAx72x4xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F34)	T cells	KO
SA333068	Norm. Area 088_0009_01A_R_TM23xWTxTAx72x5xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F35)	T cells	KO
SA333069	Norm. Area 088_0009_01A_R_TM24xKOxTAx72x1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F36)	T cells	KO
SA333070	Norm. Area 088_0009_01D_R_TM48xWTxCMLxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F221)	T cells	KO
SA333071	Norm. Area 088_0009_01D_R_TM47xWTxCMLxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F220)	T cells	KO
SA333072	Norm. Area 088_0009_01D_R_TM41xKOxCMLxTFx4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F214)	T cells	KO
SA333073	Norm. Area 088_0009_01A_R_TM38xKOxCMLxTFx1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F49)	T cells	KO
SA333074	Norm. Area 088_0009_01A_R_TM39xKOxCMLxTFx2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F50)	T cells	KO
SA333075	Norm. Area 088_0009_01D_R_TM43xKOxCMLxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F215)	T cells	KO
SA333076	Norm. Area 088_0009_01D_R_TM43xKOxCMLxTFx5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F216)	T cells	KO
SA333077	Norm. Area 088_0009_01D_R_TM46xKOxCMLxTAx24x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F219)	T cells	KO
SA333078	Norm. Area 088_0009_01D_R_TM45xKOxCMLxTAx24x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F218)	T cells	KO
SA333079	Norm. Area 088_0009_01D_R_TM44xKOxCMLxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F217)	T cells	KO
SA333080	Norm. Area 088_0009_01A_R_TM21xWTxTAx72x3xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F33)	T cells	KO
SA333081	Norm. Area 088_0009_01A_R_TM20xWTxTAx72x2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F32)	T cells	KO
SA333082	Norm. Area 088_0009_01D_R_TM51xWTxCMLxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F224)	T cells	KO
SA333083	Norm. Area 088_0009_01D_R_TM50xWTxCMLxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F223)	T cells	KO
SA333084	Norm. Area 088_0009_01A_R_TM10xKOxTFx1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F22)	T cells	KO

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Collection:

Collection ID:	CO003199
Collection Summary:	Tcells from miR142 WT and KO BCR ABL mice were isolated and subjected to resting or 24h activation.
Sample Type:	T-cells
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR003215
Treatment Summary:	CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.

Treatment ID:

TR003215

Treatment Summary:

CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.

Sample Preparation:

Sampleprep ID:	SP003212
Sampleprep Summary:	Metabolite extraction on T cells were performed using 70% acetonitrile/methanol and 30% water. The metabolite extracts were subjected to untargeted metabolomics using HILIC and reversed phase chromatographies in positive and negative modes.
Processing Storage Conditions:	4℃
Extraction Method:	Polar solvent extraction
Extract Storage:	-80℃
Sample Derivatization:	none

Combined analysis:

Analysis ID	AN005056	AN005057	AN005058	AN005059
Analysis type	MS	MS	MS	MS
Chromatography type	Reversed phase	Reversed phase	HILIC	HILIC
Chromatography system	Thermo Dionex Ultimate 3000	Thermo Dionex Ultimate 3000	Thermo Dionex Ultimate 3000	Thermo Dionex Ultimate 3000
Column	Thermo Hypersil Gold C18 (150x 2.1mm, 3uM)	Thermo Hypersil Gold C18 (150x 2.1mm, 3uM)	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type	ESI	ESI	ESI	ESI
MS instrument type	Orbitrap	Orbitrap	Orbitrap	Orbitrap
MS instrument name	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Ion Mode	POSITIVE	NEGATIVE	POSITIVE	NEGATIVE
Units	Peak area	Peak area	Peak area	Peak area

Chromatography:

Chromatography ID:	CH003819
Chromatography Summary:	Reversed phase chromatography
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Thermo Hypersil Gold C18 (150x 2.1mm, 3uM)
Column Temperature:	45
Flow Gradient:	0%A for 1 min, 50% B for 4 min, 98%B for 3 min, 0% A for 5min
Flow Rate:	0.3ml/min
Injection Temperature:	4
Solvent A:	Water, 0.1% formic acid
Solvent B:	Acetonitrile, 0.1% formic acid
Transferline Temperature:	275
Target Sample Temperature:	4
Chromatography Type:	Reversed phase

Chromatography ID:	CH003820
Chromatography Summary:	HILIC chromatography
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:	45
Flow Gradient:	99%B for 1min, 85%B for 2min, 75%B for 3 min, 30%B for 3 min, 99%B for 5 min
Flow Rate:	0.35ml/min
Injection Temperature:	4
Solvent A:	10mM ammonium acetate, 95% Water, 5% Acetonitrile, 0.1% acetic acid
Solvent B:	10mM ammonium acetate, 95% Acetonitrile, 95% Water, 0.1% acetic acid
Transferline Temperature:	275
Target Sample Temperature:	4
Chromatography Type:	HILIC

MS:

MS ID:	MS004794
Analysis ID:	AN005056
Instrument Name:	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:	POSITIVE

MS ID:	MS004795
Analysis ID:	AN005057
Instrument Name:	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:	NEGATIVE

MS ID:	MS004796
Analysis ID:	AN005058
Instrument Name:	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:	POSITIVE

MS ID:	MS004797
Analysis ID:	AN005059
Instrument Name:	Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:	NEGATIVE