Summary of Study ST002211

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001412. The data can be accessed directly via it's Project DOI: 10.21228/M86M67 This work is supported by NIH grant, U2C- DK119886.

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Study IDST002211
Study TitleEffects of Medwakh Smoking on Oxidative Stress and Inflammation Among Youth in UAE using Liquid chromatography-mass spectrometry
Study SummaryOxidative stress and inflammation are closely related, and inter-dependent pathophysiological processes. Oxidative stress plays a key role in the pathophysiology of several chronic inflammatory conditions that causes cellular alterations such as lipid damage, increase of cell permeability, apoptosis/cell death, alteration in growth factors, and signaling pathways.An imbalance between the production of reactive oxygen species (ROS) and their neutralization by the antioxidant system results in the onset of oxidative stress which subsequently leads to the release of chronic inflammatory mediators such as pro-inflammatory cytokines, which causes damages to DNA, proteins, and lipids.LC-MSMS is widely used for non-targeted and non-invasive metabolomics of biological samples including saliva in a variety of conditions such as inflammatory conditions, cancers, diabetes, etc. However, to the best of our knowledge, there are currently no metabolomics-based studies identifying salivary biomarkers associated with inflammation and oxidative stress utilizing HPLC-ESI-QTOF-MS. Therefore, in this study, we aim to discover the biochemical and physiological changes induced by medwak smoking and explore the metabolomics profile of medwak smokers with a focus on the markers of oxidative stress and inflammation in comparison to nonsmokers.
Institute
Sharjah Institute for Medical Research
DepartmentWound Healing and Oral Diagnostics
LaboratorySharjah Institute for Medical Research
Last NameK.G
First NameAghila Rani
AddressSharjah, UAE
Emailanair@sharjah.ac.ae
Phone0562206252
Submit Date2022-06-20
Num Groups2
Total Subjects60
Num Males60
Study CommentsNA
PublicationsNA
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2024-01-01
Release Version1
Aghila Rani K.G Aghila Rani K.G
https://dx.doi.org/10.21228/M86M67
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003615
Analysis type MS
Chromatography type Reversed phase
Chromatography system Bruker Elute
Column Hamilton Intensity Solo 2 C18
MS Type ESI
MS instrument type QTOF
MS instrument name timsTOF
Ion Mode POSITIVE
Units AU

Chromatography:

Chromatography ID:CH002670
Chromatography Summary:uHPLC C18 Reversed phase
Instrument Name:Bruker Elute
Column Name:Hamilton Intensity Solo 2 C18
Column Temperature:35
Flow Gradient:1%B to 99%B in 15 min
Flow Rate:250 uL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
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