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MB Sample ID: SA163998
Local Sample ID: | SCC-9_3-EV intensity |
Subject ID: | SU001832 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | SCC-9 and LN1 |
Cell Strain Details: | Primary tumor (SCC-9) and metastatic (LN1) oral cancer cell lines |
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Collection:
Collection ID: | CO001825 |
Collection Summary: | EVs were isolated from SCC-9 and LN1 cell cultures through differential centrifugation. Cells were cultured until 80% cell confluence in 150 mm diameter plates, washed three times with phosphate buffered saline (PBS) and further cultivated for 48 h in media without FBS, at 37°C and 5% CO2. After serum deprivation treatment, the conditioned media (200 mL) was collected and centrifuged at 200 x g for 5 min, 2,000 x g for 15 min, 3,500 x g for 30 min and 10,000 x g for 90 min. The cleared supernatant was further ultracentrifuged at 100,000 x g for 90 min at 4°C and vesicle-containing pellets were washed with PBS by ultracentrifugation for 1.5 h at the same speed. The samples were stored at -80°C until further use. |
Sample Type: | Keratinocytes |
Storage Conditions: | -80℃ |