Return to study ST001755 main page
MB Sample ID: SA163998
| Local Sample ID: | SCC-9_3-EV intensity |
| Subject ID: | SU001832 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | SCC-9 and LN1 |
| Cell Strain Details: | Primary tumor (SCC-9) and metastatic (LN1) oral cancer cell lines |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001825 |
| Collection Summary: | EVs were isolated from SCC-9 and LN1 cell cultures through differential centrifugation. Cells were cultured until 80% cell confluence in 150 mm diameter plates, washed three times with phosphate buffered saline (PBS) and further cultivated for 48 h in media without FBS, at 37°C and 5% CO2. After serum deprivation treatment, the conditioned media (200 mL) was collected and centrifuged at 200 x g for 5 min, 2,000 x g for 15 min, 3,500 x g for 30 min and 10,000 x g for 90 min. The cleared supernatant was further ultracentrifuged at 100,000 x g for 90 min at 4°C and vesicle-containing pellets were washed with PBS by ultracentrifugation for 1.5 h at the same speed. The samples were stored at -80°C until further use. |
| Sample Type: | Keratinocytes |
| Storage Conditions: | -80℃ |
