Summary of Study ST002225
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001418. The data can be accessed directly via it's Project DOI: 10.21228/M8F409 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002225 |
| Study Title | Time sensitive contribution of the BCAA catabolism to the TCA cycle carbons in HK2, 786-O, OS-RC-2 and RFX-631 |
| Study Summary | The objective of this experiment is to test the contribution of the branched chain amino acids catabolism to the carbons used in the TCA cycle. To test this hypothesis, we incubated human renal epithelial cells (HK2) and ccRCC cell lines (786-O, 786-M1A, OS-RC-2, OS-LM1, RFX-631) with 13C6-leucine and 13C6-isoleucine in Plasmax media for 10 mins, 1 hour and 3 hours. Data were generated from 5 independent cultures. This is Part 4 of the study and the experiment number is MS52. |
| Institute | CECAD Research Center |
| Last Name | Yang |
| First Name | Ming |
| Address | Joseph-Stelzmann-Straße 26, Köln, Koeln, 50931, Germany |
| ming.yang@uni-koeln.de | |
| Phone | 4922147884306 |
| Submit Date | 2022-07-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-08-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Cell line |
|---|---|---|
| SA212194 | MS52-14 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212195 | MS52-15 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212196 | MS52-17 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212197 | MS52-18 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212198 | MS52-13 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212199 | MS52-16 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212188 | MS52-54 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212189 | MS52-52 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212190 | MS52-53 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212191 | MS52-51 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212192 | MS52-50 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212193 | MS52-49 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212200 | MS52-88 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212201 | MS52-89 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212202 | MS52-85 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212203 | MS52-87 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212204 | MS52-90 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212205 | MS52-86 | 786-M1A | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212212 | MS52-07 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212213 | MS52-11 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212214 | MS52-10 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212215 | MS52-09 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212216 | MS52-08 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212217 | MS52-12 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212206 | MS52-43 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212207 | MS52-48 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212208 | MS52-45 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212209 | MS52-46 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212210 | MS52-47 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212211 | MS52-44 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212218 | MS52-84 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212219 | MS52-79 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212220 | MS52-80 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212221 | MS52-81 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212222 | MS52-83 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212223 | MS52-82 | 786-O | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212230 | MS52-01 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212231 | MS52-02 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212232 | MS52-06 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212233 | MS52-05 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212234 | MS52-03 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212235 | MS52-04 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212224 | MS52-42 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212225 | MS52-41 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212226 | MS52-37 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212227 | MS52-40 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212228 | MS52-38 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212229 | MS52-39 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212236 | MS52-78 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212237 | MS52-76 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212238 | MS52-77 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212239 | MS52-74 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212240 | MS52-75 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212241 | MS52-73 | HK2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212248 | MS52-28 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212249 | MS52-25 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212250 | MS52-29 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212251 | MS52-30 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212252 | MS52-26 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212253 | MS52-27 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212242 | MS52-63 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212243 | MS52-64 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212244 | MS52-65 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212245 | MS52-66 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212246 | MS52-62 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212247 | MS52-61 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212254 | MS52-102 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212255 | MS52-97 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212256 | MS52-98 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212257 | MS52-99 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212258 | MS52-100 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212259 | MS52-101 | OSLM1B | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212266 | MS52-23 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212267 | MS52-24 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212268 | MS52-20 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212269 | MS52-19 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212270 | MS52-21 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212271 | MS52-22 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212260 | MS52-55 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212261 | MS52-58 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212262 | MS52-57 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212263 | MS52-56 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212264 | MS52-60 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212265 | MS52-59 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212272 | MS52-93 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212273 | MS52-94 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212274 | MS52-96 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212275 | MS52-91 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212276 | MS52-95 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212277 | MS52-92 | OSRC2 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:3 hours |
| SA212284 | MS52-36 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212285 | MS52-32 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212286 | MS52-33 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212287 | MS52-34 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212288 | MS52-31 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212289 | MS52-35 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:10 mins |
| SA212278 | MS52-72 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212279 | MS52-69 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212280 | MS52-70 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
| SA212281 | MS52-71 | RFX631 | Treatment:13C6-leucine+13C6-isoleucine | Treatment duration:1 hour |
