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MB Sample ID: SA254482
| Local Sample ID: | WTFAST3 |
| Subject ID: | SU002622 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004155 | AN004156 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | SHIMADZU UHPLC Nexera LC-30A | SHIMADZU UHPLC Nexera LC-30A |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative intensity | Relative intensity |
MS:
| MS ID: | MS003902 |
| Analysis ID: | AN004155 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | mass spectrometric analysis was performed using a Q Exactive mass spectrometer (Thermo Scientific). ESI source conditions were as follows: Heater Temp 300 °C, Sheath Gas Flow rate 45 arb, Aux Gas Flow Rate15 arb, Sweep Gas Flow Rate 1arb, Spray voltage 3.0KV, Capillary Temp 350 °C, S-Lens RF Level 50%, MS1scan ranges: 200-1800. The mass-charge ratio of lipid molecules and lipid fragments were obtained by collecting 10 fragment maps (MS 2scan, HCD) after each fullscan. The resolution of MS1 at M/Z 200 was 70,000 and that of MS2 at M/Z 200 was 17,500. LipidSearch was used for peak identification, peak extraction and lipid molecules identification (secondary identification). The main parameters were precursor tolerance: 5 ppm, Product Tolerance: 5 ppm, and Product Ion Threshold: 5%. The obtained data were subjected to quality control for subsequent data lipid difference analysis. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003903 |
| Analysis ID: | AN004156 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | mass spectrometric analysis was performed using a Q Exactive mass spectrometer (Thermo Scientific). ESI source conditions were as follows: Heater Temp 300 °C, Sheath Gas Flow rate 45 arb, Aux Gas Flow Rate15 arb, Sweep Gas Flow Rate 1arb, Spray voltage 3.0KV, Capillary Temp 350 °C, S-Lens RF Level 50%, MS1scan ranges: 200-1800. The mass-charge ratio of lipid molecules and lipid fragments were obtained by collecting 10 fragment maps (MS 2scan, HCD) after each fullscan. The resolution of MS1 at M/Z 200 was 70,000 and that of MS2 at M/Z 200 was 17,500. LipidSearch was used for peak identification, peak extraction and lipid molecules identification (secondary identification). The main parameters were precursor tolerance: 5 ppm, Product Tolerance: 5 ppm, and Product Ion Threshold: 5%. The obtained data were subjected to quality control for subsequent data lipid difference analysis. |
| Ion Mode: | NEGATIVE |
