Summary of Study ST002522

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001624. The data can be accessed directly via it's Project DOI: 10.21228/M8S99J This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002522
Study TitleLipidomics study on the effect of LBP protein on hepatic lipid composition in mice
Study SummaryStress elevates the formation of ROS and lipid peroxidation, which induce lipid droplets (LDs) accumulation and adverse metabolic disturbance. Here, we explored the novel role of Lipopolysaccharide-binding protein (LBP) as an anti-oxidant, which can capture unsaturated triglyceride (TG) into LDs to avoid lipid peroxidation. Oxidative stress upregulates LBP level and promotes LDs growth via the LBP/TG phase transition. Upon N-Acetyl-L-cysteine (NAC) elimination of ROS, LBP is exported from LD along with PRDX4, resulting in an increase in phospholipid synthesis. Chronic stress causes LBP upregulation and leads to obesity, which can be rescued by NAC treatment in vivo. These results support that LBP maintains homeostasis by coupling lipid metabolism and redox signal, which provides insights into redox medicine that mitigate stress-induced metabolic dysfunction. Hepatic lipidomics in overexpressed LBP and WT mice treated with NAC after 24h fasting
Institute
University of Science and Technology of China
DepartmentDepartment of Endocrinology and Laboratory for Diabetes
LaboratoryThe First Affiliated Hospital of USTC, Division of Life Sciences and Medicine
Last NameZhang
First NameQilun
AddressLujiang road no.17
Emailzql66666@mail.ustc.edu.cn
Phone+8618356507293
Submit Date2023-03-21
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-03-21
Release Version1
Qilun Zhang Qilun Zhang
https://dx.doi.org/10.21228/M8S99J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001624
Project DOI:doi: 10.21228/M8S99J
Project Title:LBP resists hepatic oxidative stress by regulating LD homeostasis
Project Summary:Stress elevates the formation of ROS and lipid peroxidation, which induce lipid droplets (LDs) accumulation and adverse metabolic disturbance. Here, we explored the novel role of Lipopolysaccharide-binding protein (LBP) as an anti-oxidant, which can capture unsaturated triglyceride (TG) into LDs to avoid lipid peroxidation. Oxidative stress upregulates LBP level and promotes LDs growth via the LBP/TG phase transition. Upon N-Acetyl-L-cysteine (NAC) elimination of ROS, LBP is exported from LD along with PRDX4, resulting in an increase in phospholipid synthesis. Chronic stress causes LBP upregulation and leads to obesity, which can be rescued by NAC treatment in vivo. These results support that LBP maintains homeostasis by coupling lipid metabolism and redox signal, which provides insights into redox medicine that mitigate stress-induced metabolic dysfunction.
Institute:Department of Endocrinology and Laboratory for Diabetes, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China
Last Name:Zhang
First Name:Qilun
Address:Lujiang road no.17
Email:zql66666@mail.ustc.edu.cn
Phone:+8618356507293

Subject:

Subject ID:SU002622
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment
SA254474KIHFD3LBPKI/KI HFD16W
SA254475KIHFD2LBPKI/KI HFD16W
SA254476KIHFD1LBPKI/KI HFD16W
SA254477KIND2LBPKI/KI ND
SA254478KIND1LBPKI/KI ND
SA254479KIND3LBPKI/KI ND
SA254480WTFAST2Wild-type FAST24H
SA254481WTFAST1Wild-type FAST24H
SA254482WTFAST3Wild-type FAST24H
SA254483WTFASTNAC1Wild-type FAST24HNAC3H
SA254484WTFASTNAC2Wild-type FAST24HNAC3H
SA254485WTFASTNAC3Wild-type FAST24HNAC3H
SA254486WTHFD1Wild-type HFD16W
SA254487WTHFD2Wild-type HFD16W
SA254488WTHFD3Wild-type HFD16W
SA254489WTND2Wild-type ND
SA254490WTND3Wild-type ND
SA254491WTND1Wild-type ND
Showing results 1 to 18 of 18

Collection:

Collection ID:CO002615
Collection Summary:Quick-frozen fresh tissue with liquid nitrogen
Sample Type:Liver

Treatment:

Treatment ID:TR002634
Treatment Summary:WT and LBPKI/KI mice were fed with normal diet(ND) and high-fat diet(HFD) for 16 weeks respectively. WT mice were fasted for 24 hours or treated with NAC for 3h after a 24-hour fast.

Sample Preparation:

Sampleprep ID:SP002628
Sampleprep Summary:Lipids were extracted according to MTBE method. Briefly, a 200-µL volume of water was added to 30 mg sample and vortexed for 5 s. Subsequently, 240 µL of precooling methanol was added and the mixture vortexed for 30 s. After that, 800 µL of MTBE was added and the mixture was ultrasound 20 min at 4℃ followed by sitting still for 30 min at room temperature. The solution was centrifuged at 14000g for 15min at 10℃ and the upper organic solvent layer was obtained and dried under nitrogen.

Combined analysis:

Analysis ID AN004155 AN004156
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system SHIMADZU UHPLC Nexera LC-30A SHIMADZU UHPLC Nexera LC-30A
Column Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Relative intensity Relative intensity

Chromatography:

Chromatography ID:CH003075
Instrument Name:SHIMADZU UHPLC Nexera LC-30A
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:0-2 minutes, B was maintained at 30%; 2–25 min, B changed linearly from 30% to 100%; 25–35 min, B maintained at 30%.
Flow Rate:300 μL/min
Solvent A:60% acetonitrile/40% water; 0.1% formic acid; 0.1Mm ammonium formate
Solvent B:10% acetonitrile/90% isopropanol; 0.1% formic acid; 0.1Mm ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS003902
Analysis ID:AN004155
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:mass spectrometric analysis was performed using a Q Exactive mass spectrometer (Thermo Scientific). ESI source conditions were as follows: Heater Temp 300 °C, Sheath Gas Flow rate 45 arb, Aux Gas Flow Rate15 arb, Sweep Gas Flow Rate 1arb, Spray voltage 3.0KV, Capillary Temp 350 °C, S-Lens RF Level 50%, MS1scan ranges: 200-1800. The mass-charge ratio of lipid molecules and lipid fragments were obtained by collecting 10 fragment maps (MS 2scan, HCD) after each fullscan. The resolution of MS1 at M/Z 200 was 70,000 and that of MS2 at M/Z 200 was 17,500. LipidSearch was used for peak identification, peak extraction and lipid molecules identification (secondary identification). The main parameters were precursor tolerance: 5 ppm, Product Tolerance: 5 ppm, and Product Ion Threshold: 5%. The obtained data were subjected to quality control for subsequent data lipid difference analysis.
Ion Mode:POSITIVE
  
MS ID:MS003903
Analysis ID:AN004156
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:mass spectrometric analysis was performed using a Q Exactive mass spectrometer (Thermo Scientific). ESI source conditions were as follows: Heater Temp 300 °C, Sheath Gas Flow rate 45 arb, Aux Gas Flow Rate15 arb, Sweep Gas Flow Rate 1arb, Spray voltage 3.0KV, Capillary Temp 350 °C, S-Lens RF Level 50%, MS1scan ranges: 200-1800. The mass-charge ratio of lipid molecules and lipid fragments were obtained by collecting 10 fragment maps (MS 2scan, HCD) after each fullscan. The resolution of MS1 at M/Z 200 was 70,000 and that of MS2 at M/Z 200 was 17,500. LipidSearch was used for peak identification, peak extraction and lipid molecules identification (secondary identification). The main parameters were precursor tolerance: 5 ppm, Product Tolerance: 5 ppm, and Product Ion Threshold: 5%. The obtained data were subjected to quality control for subsequent data lipid difference analysis.
Ion Mode:NEGATIVE
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