Summary of Study ST002211
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001412. The data can be accessed directly via it's Project DOI: 10.21228/M86M67 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002211 |
| Study Title | Effects of Medwakh Smoking on Oxidative Stress and Inflammation Among Youth in UAE using Liquid chromatography-mass spectrometry |
| Study Summary | Oxidative stress and inflammation are closely related, and inter-dependent pathophysiological processes. Oxidative stress plays a key role in the pathophysiology of several chronic inflammatory conditions that causes cellular alterations such as lipid damage, increase of cell permeability, apoptosis/cell death, alteration in growth factors, and signaling pathways.An imbalance between the production of reactive oxygen species (ROS) and their neutralization by the antioxidant system results in the onset of oxidative stress which subsequently leads to the release of chronic inflammatory mediators such as pro-inflammatory cytokines, which causes damages to DNA, proteins, and lipids.LC-MSMS is widely used for non-targeted and non-invasive metabolomics of biological samples including saliva in a variety of conditions such as inflammatory conditions, cancers, diabetes, etc. However, to the best of our knowledge, there are currently no metabolomics-based studies identifying salivary biomarkers associated with inflammation and oxidative stress utilizing HPLC-ESI-QTOF-MS. Therefore, in this study, we aim to discover the biochemical and physiological changes induced by medwak smoking and explore the metabolomics profile of medwak smokers with a focus on the markers of oxidative stress and inflammation in comparison to nonsmokers. |
| Institute | Sharjah Institute for Medical Research |
| Department | Wound Healing and Oral Diagnostics |
| Laboratory | Sharjah Institute for Medical Research |
| Last Name | K.G |
| First Name | Aghila Rani |
| Address | Sharjah, UAE |
| anair@sharjah.ac.ae | |
| Phone | 0562206252 |
| Submit Date | 2022-06-20 |
| Num Groups | 2 |
| Total Subjects | 60 |
| Num Males | 60 |
| Study Comments | NA |
| Publications | NA |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003615 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Bruker Elute |
| Column | Hamilton Intensity Solo 2 C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
MS:
| MS ID: | MS003366 |
| Analysis ID: | AN003615 |
| Instrument Name: | timsTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS2 data were acquired in data-dependent acquisition using a fixed cycle time of 3s and using a scan range of 20 - 1,300 Metaboscape 4.0 was used for feature detection, retention time alignment, deisotoping and metabolite identification. |
| Ion Mode: | POSITIVE |
