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MB Sample ID: SA010739
Local Sample ID: | BM049411SC003 |
Subject ID: | SU000240 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000242 |
Sampleprep Summary: | 1) Incubated cell pellets on ice for 15 min 2) Added PBS to cells and vortex well 3) Sonicate for 1 min in ice bath 4) Added MeOH+ACN mixture to cells and vortex well 5) Sonicated for 1 min in ice bath 6) Incubated on ice for 30 min + spin for 20 min at 18K RPM 7) Combined 110 ul from each sample into a pool sample (330 ul total) 8) Split pool into two fractions of 150 ul each (hilic & C18) 9) Split remaning sample into 150 ul x 2 (hilic & C18) |