Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA010739

Local Sample ID:	BM049411SC003
Subject ID:	SU000240
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP000242
Sampleprep Summary:	1) Incubated cell pellets on ice for 15 min 2) Added PBS to cells and vortex well 3) Sonicate for 1 min in ice bath 4) Added MeOH+ACN mixture to cells and vortex well 5) Sonicated for 1 min in ice bath 6) Incubated on ice for 30 min + spin for 20 min at 18K RPM 7) Combined 110 ul from each sample into a pool sample (330 ul total) 8) Split pool into two fractions of 150 ul each (hilic & C18) 9) Split remaning sample into 150 ul x 2 (hilic & C18)

Sampleprep ID:

SP000242

Sampleprep Summary:

1) Incubated cell pellets on ice for 15 min
2) Added PBS to cells and vortex well
3) Sonicate for 1 min in ice bath
4) Added MeOH+ACN mixture to cells and vortex well
5) Sonicated for 1 min in ice bath
6) Incubated on ice for 30 min + spin for 20 min at 18K RPM
7) Combined 110 ul from each sample into a pool sample (330 ul total)
8) Split pool into two fractions of 150 ul each (hilic & C18)
9) Split remaning sample into 150 ul x 2 (hilic & C18)