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MB Sample ID: SA006530
Local Sample ID: | F06 |
Subject ID: | SU000140 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 20 weeks |
Weight Or Weight Range: | 26.8 +/- 2.2 g |
Gender: | Male |
Animal Animal Supplier: | Jackson Labs (Bar Harbor, ME) |
Animal Housing: | 8 / cage |
Animal Light Cycle: | 12 h / 12 h light/dark |
Animal Feed: | Standard chow, ad lib. |
Animal Water: | Ad lib. Access |
Species Group: | Mammal |
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Treatment:
Treatment ID: | TR000142 |
Treatment Summary: | Mice were prepared or tissue collection by one of 6 modes of anesthesia or euthanasia, then tissues were rapidly surgically collected and frozen |
Animal Anesthesia: | One of 6 methods: 1) Cervical dislocation, 2) Carbon dioxide inhalation, 3) Isoflurane overdose, 4) continuous isoflurane, 5) ketamine, 6) pentobarbital |
Animal Fasting: | 5 hours |
Animal Endp Euthanasia: | All tissue collection were terminal studies; in methods 1-3, animals were euthanized prior to tissue collection; in methods 4-6, animals were under deep anesthesia while tissues were collected. |