Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA174917

Local Sample ID:	20210513_ColmIM1_1.cdf
Subject ID:	SU001956
Subject Type:	Plant
Subject Species:	Saccharum spp

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Treatment:

Treatment ID:	TR001968
Treatment Summary:	Sugarcane cultivar SP80-3280 was grown in greenhouse conditions, at Piracicaba, São Paulo, Brazil (22°43’31’’S; 47°38’57’’W). Culms with one bud were planted in plastic trays and after acclimatization for two months, nine plants were transferred to 100 L plastic pots, containing soil (latosol). The temperature in the greenhouse was automatically adjusted to 28ºC ± 1ºC and 12/12 h light/dark photoperiod. Plants were watered to maintain soil capacity once a day, in the morning. Fertilizer was supplied once a week using a commercial fertilizer (Plant Prod®, N 15: P2O5 15: K2O 30, Plant Products CO. Canada) at the rate of 2 L per pot (4g/L). The replicates, distributed in a randomized design, were harvest at 4 month-old. During harvest, leaves were excised from the main culm and the internodes 5 and 9 from each plant, were separated and peeled from the top towards the base. Each individual internode was frozen in liquid nitrogen and kept at -80ºC. Six biological replicates from each internode were harvested.

Treatment ID:

TR001968

Treatment Summary:

Sugarcane cultivar SP80-3280 was grown in greenhouse conditions, at Piracicaba, São Paulo, Brazil (22°43’31’’S; 47°38’57’’W). Culms with one bud were planted in plastic trays and after acclimatization for two months, nine plants were transferred to 100 L plastic pots, containing soil (latosol). The temperature in the greenhouse was automatically adjusted to 28ºC ± 1ºC and 12/12 h light/dark photoperiod. Plants were watered to maintain soil capacity once a day, in the morning. Fertilizer was supplied once a week using a commercial fertilizer (Plant Prod®, N 15: P2O5 15: K2O 30, Plant Products CO. Canada) at the rate of 2 L per pot (4g/L). The replicates, distributed in a randomized design, were harvest at 4 month-old. During harvest, leaves were excised from the main culm and the internodes 5 and 9 from each plant, were separated and peeled from the top towards the base. Each individual internode was frozen in liquid nitrogen and kept at -80ºC. Six biological replicates from each internode were harvested.