Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA333174

Local Sample ID:	Norm. Area 088_0009_01A_R_TM8xWTxTAx24x3xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F20)
Subject ID:	SU003206
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR003215
Treatment Summary:	CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.

Treatment ID:

TR003215

Treatment Summary:

CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.