Summary of Study ST002298
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001472. The data can be accessed directly via it's Project DOI: 10.21228/M8FT53 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002298 |
| Study Title | NAD(P) deficiency plays an important role in the restraint-stress-induced depression in the rat model |
| Study Summary | The metabolic dysfunction or irreversible metabolic changes from stress may cause body vulnerability, potentially leading to the onset of psychiatric and non-psychiatric illnesses. Nevertheless, little is known about the biochemical events that cause depression due to stress. Our study employed open field test, plasma adrenocorticotropic hormone (ACTH) and corticosterone determination, serum biochemical analysis, quantitative PCR, immunoblotting, enzyme activity assay, and NMR-based metabolomics to analyze and identify the biochemical variations of body fluids (serum and urine) and tissues (brain, kidney, liver, lung, and spleen) in an acute restraint stress-induced rat model of depression. Our data suggested that the post-stress effects on biochemical alterations involved different biochemical pathways, including regulating the NAD(P) pool, glucose homeostasis, biosynthesis and degradation of heme, and uric acid production and metabolism. The urinary excretion of nicotinate and nicotinamide N-oxide increased significantly. Thus, we conclude that the depletion of NAD(P) precursors may occur in response to restraint stress. Our results show a close association between NAD(P) deficiency and post-stress metabolic dysfunction, which would provide a ground for developing recovery-promoting micronutrients in treating depression. |
| Institute | Anhui Science and Technology University |
| Last Name | Li |
| First Name | Jinquan |
| Address | No. 9, Donghua Road, Fengyang, Anhui Province, 233100, China |
| lijinquan@ahstu.edu.cn | |
| Phone | 86 133 2875 1890 |
| Submit Date | 2022-07-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-08-15 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002396 |
| Treatment Summary: | According to the National Institutes of Health Guide for the Care and Use of Laboratory Animals, all animals involved in this study were cared for, and protocols were reviewed and approved by the Anhui Laboratory Animal Care Committee. The specific pathogen-free (SPF) seven-week-old male Sprague Dawley (SD) rats (weight 233 ± 5 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd and used in this study. The environmental conditions were set at 21-26°C with a relative humidity of 50 ± 10% and a 12/12 h light/dark cycle. Food and tap water were provided ad libitum, and body weights were recorded daily. After one week of acclimatization, rats were randomly assigned to the groups of non-stressed control (n = 6) or the stressed (n = 7). For restraint stress, rats were individually placed in a ventilated plastic tube restrainer for 120 minutes, using a previously modified method. According to the general protocol, control rats were left undistributed in a home cage and allowed to contact each other without food and water. |
