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MB Sample ID: SA000041
| Local Sample ID: | iPSC-T2R2 |
| Subject ID: | SU000004 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | Embryo |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000004 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | Embryo |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| iPSC-T2R2 | SA000041 | FL000007 | iPSC | Mouse Cell Line |
Collection:
| Collection ID: | CO000003 |
| Collection Summary: | - |
| Sample Type: | Fibroblasts |
Treatment:
| Treatment ID: | TR000005 |
Sample Preparation:
| Sampleprep ID: | SP000003 |
| Sampleprep Summary: | Cold Methanol Extraction |
| Processing Method: | Homogenization and Solvent Removal w/ Speed Vac |
| Processing Storage Conditions: | On Ice |
| Extraction Method: | 3:1 MeOH/H2O |
| Extract Concentration Dilution: | 1,000,000 Cells/mL |
| Extract Storage: | -80C |
| Sample Resuspension: | 100 uL of 9:1 ACN/H2O with 5mM NH4OAc + 0.2% Acetic Acid |
| Cell Type: | embryonic fibroblasts, embronic stem cells, induced pluripotent stem cells |
Combined analysis:
| Analysis ID | AN000003 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6530 QTOF |
| Ion Mode | POSITIVE |
| Units | Peak height |
Chromatography:
| Chromatography ID: | CH000003 |
| Chromatography Summary: | Untargeted HILIC Method |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Pressure: | 300 Bar (Max) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-4 min - 0% B, 4-12 min - Gradient to 45% B, 12-20 min - 45% B |
| Flow Rate: | 0.25 mL/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 5 uL |
| Solvent A: | 100% water; 0.2% acetic acid; 5 mM ammonium acetate |
| Solvent B: | 90% acetonitrile/10% water; 0.2% acetic acid; 5 mM ammonium acetate |
| Analytical Time: | 20 min |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000003 |
| Analysis ID: | AN000003 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Collision Energy: | Ramped - 0.02eV/m/z + 10 eV |
| Collision Gas: | N2 |
| Dry Gas Flow: | Neb - 10 L/min, Sheath 9 L/min |
| Dry Gas Temp: | Neb/Sheath - 350C |
| Gas Pressure: | 35 PSI |
| Ionization: | Electrospray Ionization |
| Mass Accuracy: | <1.5 mDa |
| Dataformat: | .d |
| Scan Range Moverz: | 100-1700 |
