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MB Sample ID: SA000784
| Local Sample ID: | 104147 |
| Subject ID: | SU000030 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 0.25 years |
| Gender: | male |
| Animal Animal Supplier: | University of Ottawa, Canada Mary-Ellen Harper PhD, PI; |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000030 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 0.25 years |
| Gender: | male |
| Animal Animal Supplier: | University of Ottawa, Canada Mary-Ellen Harper PhD, PI; |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 104147 | SA000784 | FL000278 | cytosol | Compartment |
| 104147 | SA000784 | FL000278 | palmitoyl carnitine 9.6 uM | Skeletal Muscle Treatment |
| 104147 | SA000784 | FL000278 | 0 | Minutes |
Collection:
| Collection ID: | CO000014 |
| Collection Summary: | - |
| Sample Type: | Tissue |
| Collection Method: | isolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursors. |
| Collection Time: | 9/4/06 |
| Volumeoramount Collected: | 1 mg |
Treatment:
| Treatment ID: | TR000022 |
| Treatment Compound: | palmitic acid 2.4 uM | palmitic acid 9.6 uM | palmitoyl carnitine 9.6 uM |
Sample Preparation:
| Sampleprep ID: | SP000027 |
| Sampleprep Summary: | - |
| Sampleprep Protocol ID: | Mitochondrial Matrix Preparation |
| Extraction Method: | Extramitochondrial Buffer Preparation: The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix. Pellets were resuspended in 100 ul of buffer (140 mM KCL, 20 HEPES, 5 K2HPO4, 1 EGTA, pH 7.4 at RT) |
| Organ: | skeletal muscle |
| Organ Specification: | total |
| Cell Type: | myocyte |
| Subcellular Location: | extramitochondrial buffer |
Combined analysis:
| Analysis ID | AN000031 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Peak height |
Chromatography:
| Chromatography ID: | CH000014 |
| Instrument Name: | Agilent 6890N |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000030 |
| Analysis ID: | AN000031 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
