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MB Sample ID: SA000804
| Local Sample ID: | 332129 |
| Subject ID: | SU000031 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6/J |
| Age Or Age Range: | 0.4 years |
| Gender: | Female |
| Animal Animal Supplier: | University of Ottawa, Vivarium. |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000031 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6/J |
| Age Or Age Range: | 0.4 years |
| Gender: | Female |
| Animal Animal Supplier: | University of Ottawa, Vivarium. |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 332129 | SA000804 | FL000282 | buffer | Compartment |
| 332129 | SA000804 | FL000282 | ethanol only | Skeletal Muscle Treatment |
| 332129 | SA000804 | FL000282 | 0 | Minutes |
Collection:
| Collection ID: | CO000015 |
| Collection Summary: | - |
| Sample Type: | Tissue |
| Collection Method: | Isolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursor and inhibitors |
| Collection Time: | 4/19/07 |
| Volumeoramount Collected: | 1 mg |
Treatment:
| Treatment ID: | TR000025 |
| Treatment Compound: | ethanol only | 9 uM palmitate+inhibitors (rotenone+malonate) | 9 uM palmitate | 2 uM palmitate | 19 uM palmitate | |
Sample Preparation:
| Sampleprep ID: | SP000028 |
| Sampleprep Summary: | - |
| Sampleprep Protocol ID: | Extramitochondrial Buffer Preparation |
| Extraction Method: | The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix. |
| Organ: | skeletal muscle |
| Organ Specification: | total |
| Cell Type: | myocyte |
| Subcellular Location: | extramitochondrial buffer |
Combined analysis:
| Analysis ID | AN000032 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Peak height |
Chromatography:
| Chromatography ID: | CH000015 |
| Instrument Name: | Agilent 6890N |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000031 |
| Analysis ID: | AN000032 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
