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MB Sample ID: SA000909
Local Sample ID: | 332601 |
Subject ID: | SU000031 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 0.4 years |
Gender: | Female |
Animal Animal Supplier: | University of Ottawa, Vivarium. |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000031 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 0.4 years |
Gender: | Female |
Animal Animal Supplier: | University of Ottawa, Vivarium. |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
332601 | SA000909 | FL000298 | 0 | Minutes |
332601 | SA000909 | FL000298 | buffer | Compartment |
332601 | SA000909 | FL000298 | 19 uM palmitate | Skeletal Muscle Treatment |
Collection:
Collection ID: | CO000015 |
Collection Summary: | - |
Sample Type: | Tissue |
Collection Method: | Isolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursor and inhibitors |
Collection Time: | 4/19/07 |
Volumeoramount Collected: | 1 mg |
Treatment:
Treatment ID: | TR000025 |
Treatment Compound: | ethanol only | 9 uM palmitate+inhibitors (rotenone+malonate) | 9 uM palmitate | 2 uM palmitate | 19 uM palmitate | |
Sample Preparation:
Sampleprep ID: | SP000028 |
Sampleprep Summary: | - |
Sampleprep Protocol ID: | Extramitochondrial Buffer Preparation |
Extraction Method: | The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix. |
Organ: | skeletal muscle |
Organ Specification: | total |
Cell Type: | myocyte |
Subcellular Location: | extramitochondrial buffer |
Combined analysis:
Analysis ID | AN000032 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus III GC TOF |
Ion Mode | POSITIVE |
Units | Peak height |
Chromatography:
Chromatography ID: | CH000015 |
Instrument Name: | Agilent 6890N |
Chromatography Type: | GC |
MS:
MS ID: | MS000031 |
Analysis ID: | AN000032 |
Instrument Name: | Leco Pegasus III GC TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |